Purified anti-human HLA-A,B,C Antibody

Pricing & Availability
Clone
W6/32 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class I, MHC class I
Isotype
Mouse IgG2a, κ
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Product Citations
publications
w632_012105
HEK293 cells were transfected with RelA or empty vector and 24hrs later cell extracts harvested using a 1% CHAPS lysis buffer. Extracts were resolved by non-denaturing, non-reducing electrophoresis, transferred to nitrocellulose, and probed with a 1:500 dilution purified W6/32 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. These data document that MHC class I was upregulated in cells constitutively expressing RelA. (Data was provided by Dr. Ezra Burstein, University of Michigan Medical School, Ann Arbor, MI).
  • w632_012105
    HEK293 cells were transfected with RelA or empty vector and 24hrs later cell extracts harvested using a 1% CHAPS lysis buffer. Extracts were resolved by non-denaturing, non-reducing electrophoresis, transferred to nitrocellulose, and probed with a 1:500 dilution purified W6/32 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. These data document that MHC class I was upregulated in cells constitutively expressing RelA. (Data was provided by Dr. Ezra Burstein, University of Michigan Medical School, Ann Arbor, MI).
  • W632_Purified_072607
    HEK293 cells were transfected with RelA or empty vector and 24hrs later cell extracts harvested using a 1% CHAPS lysis buffer. Extracts were resolved by non-denaturing, non-reducing electrophoresis, transferred to nitrocellulose, and probed with a 1:500 dilution purified W6/32 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. These data document that MHC class I was upregulated in cells constitutively expressing RelA. (Data was provided by Dr. Ezra Burstein, University of Michigan Medical School, Ann Arbor, MI).
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311402 100 µg 72€
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Description

MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Activ, Block, IHC-F, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.

Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
  2. Stern P, et al. 1987. J. Immunol. 138:1088.
  3. Tran TM, et al. 2001. Immunogenetics 53:440.
  4. Barbatis C, et al. 1981. Gut 22:985.
  5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
  6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
  7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
  8. Turco MC, et al. 1988. J. Immunol. 141:2275.
  9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
  10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  11. Nagano M, et al. 2007. Blood 110:151.
  12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
  13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
  14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
  15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
  16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
  18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
  19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations
  1. Zhu Z, et al. 2022. Front Oncol. 12:814312. PubMed
  2. Gallen C, et al. 2022. Vaccines (Basel). 10:. PubMed
  3. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  4. Chen WT, et al. 2018. J Invest Dermatol. 138:1546. PubMed
  5. Jimenez-Duran G, et al. 2022. Front Immunol. 13:918551. PubMed
  6. Chou JM, et al. 2022. Front Microbiol. 13:883597. PubMed
  7. Nguyen R, et al. 2021. Cancer Immunol Immunother. 70:721. PubMed
  8. Tsuchiya N, et al. 2018. Oncoimmunology. 7:e1377872. PubMed
  9. Abdul–Salam VB, et al. 2019. Circ Res. 124:52:00. PubMed
  10. Hsiue EH, et al. 2021. Science. 371:. PubMed
  11. Andersen R, et al. 2018. Cancer Immunol Res. 0.404166667. PubMed
  12. Laing B, et al. 2010. J Thorac Cardiovasc Surg. 139:1402. PubMed
  13. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  14. Wroblewska A et al. 2018. Cell. 175(4):1141-1155 . PubMed
  15. Willimsky G, et al. 2021. Elife. 10:. PubMed
  16. Garrido C, et al. 2018. JCI Insight. 3:e120121. PubMed
  17. Takeuchi M, et al. 2010. J Mol Cell Biol. 0.179861111. PubMed
  18. Mirandola L, et al. 2017. Oncotarget. 8:74378. PubMed
  19. Sharma M, et al. 2020. Front Immunol. 11:1136. PubMed
  20. Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
  21. Parker R, et al. 2020. bioRxiv. . PubMed
  22. A A, et al. 2016. Biotechnol Bioeng. 112: 2214-27. PubMed
  23. Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
  24. Gañán-Gómez I, et al. 2022. Nat Med. . PubMed
  25. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  26. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  27. Higashi S, et al. 2022. J Cell Sci. 135:. PubMed
  28. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  29. Yumoto K, et al. 2016. Sci Rep. 6:36520. PubMed
  30. Ruiz Cuevas MV, et al. 2021. Cell Reports. 34(10):108815. PubMed
  31. Tanaka Y, et al. 2020. Sci Rep. 10:17284. PubMed
  32. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  33. Han EX, et al. 2021. NPJ Regen Med. 6:40. PubMed
  34. Liu Y, et al. 2017. Clin Cancer Res. 23(2):514-522. PubMed
  35. Blees A, et al. 2017. Nature.. 10.1038/nature24627. PubMed
  36. Chen YL, et al. 2020. J Exp Med. 217:. PubMed
  37. Nöll A, et al. 2017. Proc Natl Acad Sci U S A. 114: E438 - E447. PubMed
  38. Makarkov AI, et al. 2019. NPJ Vaccines. 4:17. PubMed
  39. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  40. Neuperger P, et al. 2021. Cancers (Basel). 14:. PubMed
  41. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  42. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  43. McLoughlin R, et al. 2008. J Immunol. 181:1323. PubMed
  44. Pierini S, et al. 2020. JCI Insight. 5:00. PubMed
  45. Takahara M, et al. 2008. J Leukoc Biol. 83:742. PubMed
  46. Pettmann J, et al. 2021. eLife. 10:00. PubMed
  47. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  48. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  49. Carlsten M, et al. 2019. Oncoimmunology. 8:e1534664. PubMed
  50. Nosaka K, et al. 2021. Anticancer Res. 41:4741. PubMed
  51. Jasmer B, et al. 2017. Oncotarget. 8:108643. PubMed
  52. Chen HC, et al. 2017. Immunol Cell Biol. 95:620. PubMed
  53. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
RRID
AB_314871 (BioLegend Cat. No. 311402)

Antigen Details

Structure
Ig superfamily
Distribution

All nucleated cells

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A,B,C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09/06/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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