PE anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEL-14_PE_090707
C57BL/6 mouse splenocytes stained with MEL-14 PE
  • MEL-14_PE_090707
    C57BL/6 mouse splenocytes stained with MEL-14 PE
See PE spectral data
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104407 50 µg 72,00€
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104408 200 µg 200,00€
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. García Nores GD, et al. 2018. Nat Commun. 9:1970. PubMed
  2. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  3. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  4. Ren S, et al. 2019. Oncol Rep. 41:1509. PubMed
  5. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  6. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  7. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  8. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  9. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  10. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  11. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  12. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  13. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  14. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  15. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  16. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  17. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  18. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  19. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  20. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  21. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  22. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  23. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  24. MacDonald K, et al. 2014. J Immunol. 192:3180. PubMed
  25. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  26. Hamasato E, et al. 2014. Brain Behav Immun. 11:109. PubMed
  27. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  28. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  29. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  30. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  31. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  32. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  33. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  34. Ma X, et al. 2020. Immunity. 53:1315. PubMed
  35. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  36. Guo S, et al. 2021. Frontiers in Immunology. 11:614667. PubMed
  37. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  38. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  39. Tian T, et al. 2020. Cancer Immunol Res. 660:8. PubMed
  40. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  41. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  42. Zheng B, et al. 2021. Bioact Mater. 6:3879. PubMed
RRID
AB_313094 (BioLegend Cat. No. 104407)
AB_313095 (BioLegend Cat. No. 104408)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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