PE anti-human/mouse Bcl-6 Antibody

Pricing & Availability
Clone
7D1 (See other available formats)
Other Names
B-cell lymphoma 6, BCL5, LAZ3, BCL6A, ZNF51, ZBTB27
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7D1_PE_Bcl-6_Antibody_FC_1_052413
11-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Pacific Blue™ and IgD PerCP/Cy5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown was gated on B220+ positive cells.
  • 7D1_PE_Bcl-6_Antibody_FC_1_052413
    11-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Pacific Blue™ and IgD PerCP/Cy5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown was gated on B220+ positive cells.
  • 7D1_PE_Bcl-6_Antibody_FC_2_052413
See PE spectral data
Cat # Size Price Quantity Avail. Save
358503 25 tests 100€
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358504 100 tests 260€
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Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Sequence 226-398 of murine BCL6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1,2,3.

Application References
  1. Vikstrom, et al. 2010. Science 330:1095. (WB)
  2. Kallies A, et al. 2011. Blood 117:1869. (WB)
  3. Lüthje K, et al. 2012. Nat. Immunol. 13:491. (WB)
  4. Tonti E, et al. 2012. J. Immunol. 188:3217. (ICFC)
Product Citations
  1. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
RRID
AB_2562151 (BioLegend Cat. No. 358503)
AB_2562152 (BioLegend Cat. No. 358504)

Antigen Details

Structure
Member of the BTB-POZ zinc finger family, 80 kD, homodimer, 1 BTB (POZ) domain, 6 C2H2-type zinc fingers
Distribution

Germinal center B cells, follicular helper T cells (TFH), B cell lymphomas, breast cancer cells

Function
Transcription factor, master regulator of germinal center reaction
Interaction
BLIMP-1
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Basso K and Dalla-Favera R. 2012. Immunol. Rev. 247:172.
2. Vinuesa CG, and Cyster JG, 2011. Immunity 35:671.
3. Kitano M, et al. 2011. Immunity 34:961.
4. Baumjohann D, et al. 2011. J. Immunol. 187:2089.
5. Johnston RJ, et al. 2009. Science. 325:1006.
6. Tran TH, et al. 2010. Cancer Res. 70:1711.

Gene ID
604 View all products for this Gene ID
UniProt
View information about Bcl-6 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/03/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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