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- Clone
- QA18A14 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B1, Bp35
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes were stained with anti-human CD19 APC and anti-human CD20 recombinant (clone QA18A14) PE (left), or mouse IgG1, κ PE isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 389103 | 25 tests | 68€ | ||||
| 389104 | 100 tests | 136€ | ||||
CD20, also known as B1 and Bp35, is a 33-37 kD four transmembrane spanning protein. CD20 is expressed on pre-B cells, resting B cells, and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Human CD20 transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
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AB_3068258 (BioLegend Cat. No. 389103)
AB_3068258 (BioLegend Cat. No. 389104)
Antigen Details
- Structure
- Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
- Distribution
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B cell, T cell subsets
- Function
- B cell activation
- Interaction
- CD20 associates with CD53, CD81, CD82, MHCII, CD40, CBP, BCR, and Src family kinases
- Ligand/Receptor
- Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
- Molecular Family
- CD Molecules
- Antigen References
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- Hultin LE, et al. 1993. Cytometry. 14:196-204.
- Tedder T and Engel P. 1994. Immunol Today. 15:450-4.
- Gene ID
- 931 View all products for this Gene ID
- UniProt
- View information about CD20 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD20 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD20 Recombinant Antibody | QA18A14 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD20 Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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