Pricing & Availability
Regulatory Status
Other Names
IL-17A/F Pre-coated ELISA Kit
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Product Citations
  • IL-17AF_Human_LegMax_112911
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435807 1 Pre-coated Plate 328€
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Human IL-17A/F is a heterodimeric member of the IL-17 family It is a pro-inflammatory cytokine secreted by activated Th17 cells in response to bacteria and fungi. There is great interest in IL-17 family members, not only for their role in adaptive immunity and inflammation, but also in a variety of auto-immune diseases, such as Crohn's disease, ulcerative colitis, psoriasis, multiple sclerosis, rheumatoid arthritis, asthma, and lupus.

IL-17A/F consists of the monomeric units of IL-17A and IL-17F, which share 50% amino acid sequence identity. It has been shown to be a naturally expressed and biologically active molecule. While much has been studied about IL-17A and IL-17F, it is still unclear how each distinctively induces the inflammatory response, and what role IL-17A/F also plays in it. It is known that all three cytokines are produced in different amounts, and each has different degrees of pro-inflammatory effects. All seem to interact with the IL-17RA/IL-17RC receptor complex, and signal via the NF-κB pathway to induce secretion of inflammatory cytokines and recruit granulocytes.

BioLegend's LEGEND MAX™ Human IL-17A/F ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a specific antibody is pre-coated on a 96-well strip-well plate.

BioLegend's LEGEND MAX™ Human IL-17A/F ELISA Kit is specifically designed for the accurate quantitation of Human IL-17A/F from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Human IL-17A/F Pre-coated 96-well Strip Microplate
  • Human IL-17A/F Dectection Antibody
  • Human IL-17A/F Standard
  • Matrix C (for serum and plasma samples only)
  • Avidin-HRP D
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Product Citations
  1. Dei Zotti F, et al. 2022. Front Immunol. 13:972127. PubMed
2.58 pg/mL
Standard Range
10.9-700 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Th17 cells and activated CD4 T cells
Biology Area
Immunology, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
3605 View all products for this Gene ID 112744 View all products for this Gene ID
View information about IL-17A/F on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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