LEGEND MAX™ Human Angiopoietin-2 ELISA Kit

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Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human Angiopoietin-2 Standard ranging from 312.5 pg/mL to 20,000 pg/mL.
  • LEGENDMAX_Human_Angiopoietin-2_012023
    Representative sandwich immunoassay standard curve generated using serial dilutions of LEGEND MAX™ Human Angiopoietin-2 Standard ranging from 312.5 pg/mL to 20,000 pg/mL.
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440407 1 Pre-coated Plate 444€
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Human Angiopoietin-2 is a member of the angiopoietin family of vascular growth factors and is a glycoprotein involved in angiogenesis and inflammation. It plays a vital role in embryonic and postnatal angiogenesis and is highly abundant in vascular endothelial cells. Angiopoietin-2, along with angiopoietin-1, are ligands for the receptor tyrosine kinase Tie2. It is also known to work synergistically with cytokines, such as VEGF, to promote tumor progression and associated angiogenesis. Studies have shown that it is a marker for endothelial activation. Due to its importance in inflammatory conditions, this analyte has great potential for new disease therapies.

The BioLegend LEGEND MAX™ Human Angiopoietin-2 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human angiopoietin-2 antibody. The detection antibody is a biotinylated mouse monoclonal anti-human angiopoietin-2 antibody. This kit is specifically designed for the accurate quantitation of human angiopoietin-2 in serum, plasma, cell culture supernatant, and saliva. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Human Angiopoietin-2 Pre-coated 96-well Strip Microplate
  • Human Angiopoietin-2 Detection Antibody
  • Human Angiopoietin-2 Lyophilized Standard
  • Avidin-HRP
  • Assay Buffer B
  • 20X Wash Buffer
  • Substrate Solution F
  • Stop Solution
  • Plate sealers

Product Details

Verified Reactivity


55.8 + 12.3 pg/mL
Standard Range
312.5 – 20,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Microplate shaker
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Polypropylene vials

Antigen Details

Cell Type
Endothelial cells
Biology Area
Molecular Family
Growth Factors
Gene ID
View information about Angiopoietin-2 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 03/19/2024

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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