FITC Avidin

Pricing & Availability
Regulatory Status
Other Names
Avidin-Fluorescein Isothiocyanate, Av-FITC
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Product Citations
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405101 100 µg 29€
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405102 500 µg 95€
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Avidin binds to biotin with high affinity. Avidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

10 mM HEPES, 0.5 M NaCl, pH 8.0, containing 0.08% sodium azide.
Avidin is conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
This solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

ICFC - Verified

Recommended Usage

Avidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the recommended use of this reagent is ≤0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Avidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies.

Application References
  1. Jeon OH, et al. 2007. Throb res. 119:609. PubMed
  2. Lin HY, et al. 2013. J. Virol. 87:9064. PubMed
Product Citations
  1. Jeon O, et al. 2007. Thromb Res. 119:609. PubMed
  2. Lin H, et al. 2013. J Virol. 87:9064. PubMed
  3. Quaranta M, et al. 2011. PLoS One. 6:e23442. PubMed
  4. Dalangood S, et al. 2020. Theranostics. 10:10078. PubMed

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Go To Top Version: 2    Revision Date: 01/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.


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