Loading...

Direct-Blot™ HRP anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
16B12_DB-HRP_HA.11_Antibody_WB_042017
Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
  • 16B12_DB-HRP_HA.11_Antibody_WB_042017
    Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
Compare all formats
Cat # Size Price Quantity Check Availability Save
901520 25 µL 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
901519 100 µL 256€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
  13. Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
  14. Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
  15. Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
  16. Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
  17. Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
  18. Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
  19. Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
  20. Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
  21. Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
  22. Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
  23. Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
  24. Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
  25. Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
  26. Lee B, et al. 2016. Development. 143:1721-31. PubMed
  27. Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
  28. Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
  29. Shin H, et al. 2015. Nature. 534:553-7. PubMed
  30. Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
  31. Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
  32. Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
  33. Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
  34. Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
  35. Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
  36. Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
  37. Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
  38. Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
  39. Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
  40. Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
  41. Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
  42. Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
  43. Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
  44. Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
  45. Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
  46. Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
  47. Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
  48. Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
  49. Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
  50. Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
  51. Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
  52. Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
  53. Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
  54. Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
  55. Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
  56. Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
  57. Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
  58. Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
  59. Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
  60. Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
  61. Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
  62. Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
  63. Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
  64. Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
  65. Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
  66. Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
  67. Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
  68. Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
  69. Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
  70. Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
  71. Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
  72. Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
  73. Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
  74. K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
  75. Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
  76. Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
  77. T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
  78. Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
  79. Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
  80. Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed
  81. Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
  82. Daniel JA, et al. 2017. Elife. 6:e26338. PubMed
  83. Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
Product Citations
  1. Sondo E et al. 2018. Cell chemical biology. 25(7):891-905 . PubMed
RRID
AB_2749912 (BioLegend Cat. No. 901520)
AB_2686981 (BioLegend Cat. No. 901519)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/21/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Anti-HA.11 Epitope Tag Affinity Matrix

  • Alexa Fluor® 488 anti-HA.11 Epitope Tag

  • Alexa Fluor® 594 anti-HA.11 Epitope Tag

  • Anti-HA.11 Epitope Tag

  • Biotin anti-HA.11 Epitope Tag

  • FITC anti-HA.11 Epitope Tag

  • Purified anti-HA.11 Epitope Tag

  • Alexa Fluor® 647 anti-HA.11 Epitope Tag

  • PE anti-HA.11 Epitope Tag

  • Direct-Blot™ HRP anti-HA.11 Epitope Tag

  • Ultra-LEAF™ Purified anti-HA.11 Epitope Tag

  • Brilliant Violet 421™ anti-HA.11 Epitope Tag

  • PE/Dazzle™ 594 anti-HA.11 Epitope Tag

  • PE/Cyanine7 anti-HA.11 Epitope Tag

  • Pacific Blue™ anti-HA.11 Epitope Tag

  • APC anti-HA.11 Epitope Tag

  • PerCP/Cyanine5.5 anti-HA.11 Epitope Tag

  • TotalSeq™-C1131 anti-HA.11 Epitope Tag

  • TotalSeq™-A1131 anti-HA.11 Epitope Tag

  • TotalSeq™-B1131 anti-HA.11 Epitope Tag

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account