DAB Substrate Buffer

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Regulatory Status
Other Names
Substrate Buffer
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926605 15 mL 44€
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926606 1000 mL 336€
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In the presence of peroxidase enzyme, DAB will produce a brown precipitate that is insoluble in alcohol. This product is the substrate buffer for use with DAB chromogen (Cat. Nos. 926506 and 926507).

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.


Recommended Usage

Each lot of this product is quality tested by immunohistochemistry.

Prepare the DAB solution by combining components as described in the following table.

Buffer     DAB Chromogen
5 mL   +   0.2 mL
25 mL +   1.0 mL
50 mL +   2.0 mL

Application Notes

1. Add the DAB chromogen to the DAB substrate buffer as described above
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 minutes at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount
6. DAB produces a brown precipitate with almost no background

Antigen Details

Gene ID

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Go To Top Version: 0    Revision Date: 03/04/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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