- Other Names
- Red blood cell lysis buffer, lysis buffer, CD buffer, RBC Buffer
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Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
420514 | 200 mL | 200€ |
Cell-Vive™ CD RBC Lysis Buffer 10X, GMP is a chemically-defined buffer designed to insure optimal lysis of human red blood cells (RBC) with minimal effects on leukocytes. This product is formulated without animal-derived components or preservatives, contains low levels of endotoxin (< 1EU/mL), and is tested negative for mycoplasma and microbial growths. Cell-Vive™ CD RBC lysis Buffer 10X, GMP is a 10X solution that should be diluted to 1X before use. This GMP product is for research and further ex vivo bioprocessing use only.
Product DetailsProduct Details
- Formulation
- Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water
- Preparation
- Chemically-Defined, no preservatives. Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. Warm the 1X solution to room temperature before use.
- Storage & Handling
- Store between 2°C-8°C
- Recommended Usage
-
Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X solution that should be diluted to 1X using water. 2 ml of 1X Cell-Vive™ CD RBC Lysis Buffer, GMP is enough to lyse up to 100 μl of whole blood.
- Application Notes
-
Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is recommended for the lysis of human red blood cells. This buffer has a chemically-defined formulation without animal-derived components, it contains low levels of endotoxin (< 1EU/mL), and it is tested negative for mycoplasma and microbial growths. Use Cell-Vive™ CD RBC Lysis Buffer (10X), GMP for highly sensitive assays.
Cell-Vive™ CD RBC Lysis Buffer (10X), GMP is a 10X formulation that contains no preservatives, handle under aseptic conditions as needed.
- Additional Product Notes
-
Human Red Blood Cells lysis protocol
- Dilute the Cell-Vive™ CD RBC Lysis Buffer (10X), GMP to 1X working concentration with deionized water. Warm the 1X solution to room temperature before use.
- Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 μl of human whole blood.
- Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes.
- Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing the pellet.
- Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell-Vive™ CD Cell Staining Buffer, GMP Cat. No. 420508), and wash 1X.
- Resuspend and proceed with further procedures.
Antigen Details
- Gene ID
- NA
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