- 145-2C11 (See other available formats)
- Other Names
- CD3ε, T3, CD3
- Armenian Hamster IgG
- Ave. Rating
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- Product Citations
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CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunocytochemistry using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32, and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Application References
- Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
- Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
- Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
- Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
- Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
- Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
- Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
- Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
- Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
- Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
- Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
- Ko SY, et al. 2005. J. Immunol. 175:3309.
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
- Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
- Xiao S, et al. 2007. J. Exp. Med. 204:1691.
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
- Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
- Guo Y, et al. 2008. Blood 112:480. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091.
- Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
- Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
- Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
- de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
- Schulteis RD, et al. 2008. Blood 295:G273. PubMed
- Qi Q, et al. 2009. Blood 114:564. PubMed
- Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
- Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
- Yan J, et al. 2014. Vaccine. 32:2833. PubMed
- Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
- Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
- Product Citations
AB_10898314 (BioLegend Cat. No. 100335)
AB_2562556 (BioLegend Cat. No. 100341)
AB_11203705 (BioLegend Cat. No. 100336)
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Thymocytes (differentiation dependent), mature T cells, NK-T cells
- TCR signal transduction, T cell activation, antigen recognition
- Peptide antigen/MHC-complex
- Cell Type
- NKT cells, T cells, Thymocytes, Tregs
- Biology Area
- Molecular Family
- CD Molecules, TCRs
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.
- Gene ID
- 12501 View all products for this Gene ID
- View information about CD3epsilon on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
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