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Alexa Fluor® 594 anti-rat IgG2a Antibody

Pricing & Availability
Clone
MRG2a-83 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Mouse IgG
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Product Citations
publications
MRG2a-83_IgG20_Antibody_1_062323
C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD8a (clone 53-6.7), 10 µg/mL of Alexa Fluor® 647 anti-CD20 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2a (clone MRG2a-83) (red) for two hours at room temperature. The image was captured by 10X objective.
  • MRG2a-83_IgG20_Antibody_1_062323
    C57BL/6 frozen mouse spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/mL of purified anti-mouse CD8a (clone 53-6.7), 10 µg/mL of Alexa Fluor® 647 anti-CD20 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 594 anti-rat IgG2a (clone MRG2a-83) (red) for two hours at room temperature. The image was captured by 10X objective.
  • MRG2a-83_IgG20_Antibody_2_062323
    HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with MeOH for 10 minutes, and blocked with 5% FBS for 1 hour. Cells were then stained with 5.0 µg/mL of purified rat anti-histone H3 phosphorylated (Ser28) (Cat. No. 641002) overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG2a (clone MRG2a-83) at 2.5 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801). The images were captured with a 40X objective. Scale bar = 50 µm
Compare all formats See Alexa Fluor® 594 spectral data
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407509 100 µg 188€
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Description

The MRG2a-83 monoclonal antibody reacts with rat immunoglobulin G2a (IgG2a) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRG2a-83 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For immunohistochemistry, a concentration range of 2-5 μg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References
  1. Inoue S, et al. 2006. Cancer Research 66:7741.
RRID
AB_2650845 (BioLegend Cat. No. 407509)

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Go To Top Version: 2    Revision Date: 06/21/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-rat IgG2a

  • Biotin anti-rat IgG2a

  • FITC anti-rat IgG2a

  • PE anti-rat IgG2a

  • Alexa Fluor® 594 anti-rat IgG2a

  • Alexa Fluor® 647 anti-rat IgG2a

  • Alexa Fluor® 488 anti-rat IgG2a
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