- Clone
- 500A2 (See other available formats)
- Other Names
- CD3ε, T3, CD3
- Isotype
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 splenocytes were stained with CD19 APC/Cyanine7 and CD3ε (clone 500A2) Alexa Fluor® 488 (left) or Syrian Hamster IgG isotype control (right) Alexa Fluor® 488. -
Balb/C mouse frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 488 anti-mouse CD3 Antibody (clone 500A2, green), Alexa Fluor® 594 anti-mouse CD11b Antibody (clone M1/70, red) and Alexa Fluor® 647 anti-mouse/human CD45R/B220 Antibody (clone RA3-6B2, blue) overnight at 4°C. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 152321 | 25 µg | 84€ | ||||
| 152322 | 100 µg | 152€ | ||||
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- Mouse T cell receptor.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
-
Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
- Application References
-
- Miescher GC, et al. 1989. Immunol. Lett. 23:113.
- RRID
-
AB_2783123 (BioLegend Cat. No. 152321)
AB_2783124 (BioLegend Cat. No. 152322)
Antigen Details
- Structure
- Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
- Distribution
-
Thymocytes (differentiation dependent), mature T cells and NK-T cells.
- Function
- TCR signal transduction, T cell activation and antigen recognition.
- Interaction
- T-cell receptor (TCR).
- Ligand/Receptor
- Peptide antigen/MHC-complex.
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- CD Molecules, TCRs
- Antigen References
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1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263. - Gene ID
- 12501 View all products for this Gene ID
- UniProt
- View information about CD3e on UniProt.org
Related FAQs
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