BioLegend provides a wide selection of antibodies validated and quality-control tested for a variety of applications including Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), or Chromatin IP (ChIP). Targets include a wide selection of cell biology proteins including cell signaling, cell structure, cell development and differentiation, cell proliferation and growth, cell cycle, epigenetic and transcriptional regulators, cell adhesion, and cell death proteins, as well as epitope tags.


Application Protocols


BioLegend’s Commitment to Antibody Quality

 

To ensure the highest quality of products, BioLegend’s cell biology antibodies undergo extensive validation processes, which include:

 

  • Testing on knockout (KO) or knockdown (KD) cell lysates (CRISPR/Cas9 or siRNA), and negative control cell lines to confirm antibody specificity.
  • Testing on multiple species (human, mouse, rat, yeast, bacteria, zebrafish) whenever applicable, to determine antibody cross-reactivity.
  • Side-by-side comparison testing with competitor’s antibody to ensure sensitivity.
  • Side-by-side comparison testing of lots with internal controls for lot-to-lot consistency.
  • Clones for one application (such as WB or ChIP) further validated in multiple applications (such as microscopy, flow cytometry) to provide the user the flexibility of supplementing their data with additional assays specific to their research.
  • Optimization of antibody suggested use by a titration range.

Go-ChIP-Grade™ Protein G Enzymatic Kit

BioLegend offers a variety of products to help perform effective ChIP assays. We offer high-quality Go-ChIP-Grade™ Purified Antibodies for ChIP assays directed toward diverse targets including histone modifications, chromatin-associated proteins, and transcription factors. We also offer a Go-ChIP-Grade™ Protein G Enzymatic Kit for a quicker, easier, and more efficient way to perform ChIP assays.

 


 

Direct-Blot™ HRP Antibodies

When performing western blots, it is common to use a secondary antibody to detect the proteins of interest. To help you perform western blots with ease, BioLegend offers Direct-Blot™ primary antibodies directly conjugated to horseradish peroxidase (HRP).


 

Western-Ready™ ECL Substrate

 

  • ECL substrate with high sensitivity
  • A luminol-based detection system
  • Suitable for CCD imager and film
  • Available in two sizes for 10 or 100 mini-blots
  • Signal duration up to 4 hours

 

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Prime-Step™ Prestained Broad Range Protein Ladder

The Prime-Step™ Prestained Broad Range Protein Ladder is a three-color protein standard with 10 prestained chromophore-conjugated recombinant proteins covering a wide range of molecular weights, from 6.5-270 kDa. It is ideal for monitoring protein separation during SDS-PAGE, confirming WB transfer on membranes, and estimating protein sizes.

data image

 

Cell Biology Antibody Sampler Kits

BioLegend offers Cell Biology Antibody Sampler Kits as a convenient and economical way to test multiple targets. These kits target various protein groups including epitope tags, HDACs, IRFs, and many others…

Current Kits include:

  • E. coli RNA Polymerase Antibody Sampler Kit
  • Epitope Tag Big Motif Antibody Sampler Kit
  • Epitope Tag Big Motif Antibody Sampler Kit
  • HDAC Antibody Sampler Kit
  • IRF Antibody Sampler Kit

 

Below are reviews from customers collected at BioLegend and other external sites for our Cell Biology products. To browse and search all the reviews, visit our Reviews Library.

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BioLegend's Cell Biology products have been extensively published. Browse and search the full Publication Library.

Product Authors Journal Year Vol/Page Pubmed
Normal Serum Block Ghanbari L, et al. Nat Commun 2019 10:1500 Link
DC-anti-14-3-3 ζ Nakav S, et al. Am J Respir Cell Mol Biol 2011 46:313 Link
DC-anti-14-3-3 ζ/δ Johnson J, et al. Proc Natl Acad Sci U S A 2006 103:19575 Link
DC-anti-14-3-3 ζ/δ Liu B, et al. PLoS One 2012 7:e44701 Link
Purified anti-14-3-3 ε Cloutier A, et al. Sci Rep 2018 8:2206 Link
DC-anti-a-Adducin Kalfa T, et al. Blood 2006 108:3637 Link
Purified anti-ABCA3 Sun-Wada G, et al. J Biol Chem 2003 278:44843-44851 Link
Purified anti-ABCA3 Mishra A, et al. J Cell Sci 2011 124:657-668 Link
Purified anti-Acetylated Lysine Kuo H, et al. Sci Signal 2010 3:ra9 Link
Purified anti-Acetylated Lysine Han S, et al. Nat Commun 2017 8:14568 Link
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