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Spark Blue™ 574 Streptavidin

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Regulatory Status
RUO
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Streptavidin_SparkBlue574_Antibody_050423
Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Blue™ 574 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Blue™ 574 and anti-human CD19 APC (right).
  • Streptavidin_SparkBlue574_Antibody_050423
    Human peripheral blood lymphocytes were stained with biotinylated anti-human CD3, followed by SAV-Spark Blue™ 574 and anti-human CD19 APC (left) or biotinylated mouse IgG1, κ isotype control, followed with SAV-Spark Blue™ 574 and anti-human CD19 APC (right).
See Spark Blue™ 574 spectral data
Cat # Size Price Quantity Check Availability Save
405355 25 µg 85€
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405356 100 µg 203€
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Description

Streptavidin is a 52.8 kDa tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used to detect biotinylated proteins in a wide range of applications.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
Streptavidin is conjugated with Spark Blue™ 574 under optimal conditions
Concentration
0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin- Spark Blue™ 574 solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this Streptavidin-Spark Blue™ 574 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Spark Blue™ 574 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 574 has a maximum excitation of 506 nm and a maximum emission of 574 nm.

Excitation Laser
Blue Laser (488 nm)

Related FAQs

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Go To Top Version: 1    Revision Date: 04/19/2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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