Recombinant Mouse IFNAR2-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
IFNABR, IFN-R-2, IFNARB, IFN-R, IFN-Alpha-REC
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Product Citations
publications
RECOM_-Mouse-IFNAR2_1_CF
Recombinant mouse IFNAR2-Fc chimera binds to immobilized recombinant mouse IFN-α (Cat. No. 752802) in a dose-dependent manner. The ED50 for this effect is 0.18 – 0.9 µg/mL.
  • RECOM_-Mouse-IFNAR2_1_CF
    Recombinant mouse IFNAR2-Fc chimera binds to immobilized recombinant mouse IFN-α (Cat. No. 752802) in a dose-dependent manner. The ED50 for this effect is 0.18 – 0.9 µg/mL.
  • RECOM_-Mouse-IFNAR2_2_CF
    Stability Testing for Recombinant Mouse IFNAR2-Fc Chimera. Recombinant mouse IFNAR2-Fc chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their property to bind to immobilized recombinant mouse INF-α (Cat. No. 752802) in a dose-dependent manner. The ED50 for this effect is 0.18 – 0.9 µg/mL.
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568604 25 µg 276€
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568606 100 µg 508€
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Description

IFN type I IFN-α/β and type II (IFNγ) play a crucial role in innate immunity against viruses and cancer. Type I IFN receptor includes two subunits IFNAR1 and IFNR2. IFNAR1 has only one isoform, whereas IFNAR2 has three isoforms, INFAR2a, INFAR2b, and INFAR2c. INFAR2a is soluble truncated form, INFAR2b possesses a short transmembrane domain and lacks the intracellular domain, and INFAR2c has full intracellular domain. INFAR2a and INFAR2b bind to IFN type I ligands and interact also with IFNAR1, but this interaction is unable to induce signal transduction. IFNAR2 possesses two fibronectin III like domains (D1 and D2) and five putative N-glycosylation sites. The IFNAR1 has four subdomains (SD1 to SD4) and 12 possible N-glycosylation sites. IFNAR1 and IFNAR2 interact with the ligands in opposite sides in an orthogonal structure. IFN-α binds to IFNAR2 and this interaction is followed by IFNAR1 association. IFNAR1 and IFNAR2 are ubiquitously expressed and the type I IFN ligands in almost all nucleated cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IFNAR2-Fc, amino acid (Ser22-Ala242) (Accession: # O35664) with a C-terminus mIgG2a-Fc tag was expressed in CHO cells.
Molecular Mass
The 461 amino acid recombinant protein has a predicted molecular mass of approximately 51.9 kD. The DTT-reduced and non-reduced protein migrates at approximately at 70 – 80 KD and 140 - 150 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.2 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick the spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant mouse IFNAR2-Fc chimera binds to immobilized recombinant mouse IFN-α in a dose-dependent manner. The ED50 for this effect is 0.18 – 0.9 µg/mL.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Ubiquitously expressed 

Function
Innate response to intracellular microbes, antiviral, immunomodulatory, anti-tumor, and antiproliferative activities
Interaction
INFAR1
Ligand/Receptor
IFNβ, IFNε, IFNκ, IFNω and 12 subtypes of IFNα
Bioactivity
Recombinant mouse IFNAR2 binds to immobilized mouse IFN-α
Cell Type
Antigen-presenting cells, B cells, Endothelial cells, Epithelial cells, Fibroblasts, Granulocytes, Leukocytes, Lymphocytes, Macrophages, Mast cells, Mature Neurons, Microglia, Monocytes, Neurons, Neutrophils, NK cells, NKT cells, Oligodendrocytes, Osteoblasts, Osteoclasts, T cells, Th1, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity
Molecular Family
Innate Immune Signaling
Antigen References
  1. Novick D, et al. 1994, Cell. 77:391-400.
  2. De Weerd NA, et al. 2007. J Biol Chem. 282:20053-7.
  3. Strunk JJ, et al. 2008. J Mol Biol. 377:725-39.
  4. Plumlee CR, et al. 2009. J Biol Chem. 284:30058-66.
  5. Thomas C, et al. 2011. Cell. 146:621-32.
  6. Chuartzman SG, et al. 2017. PLoS One. 12:e0175413.
  7. Zanin N, et al. 2021. Front Immunol. 11:615603.
  8. Samarajiwa SA, et al. 2014. J Immunol. 192:4425-35.
  9. Hurtado-Guerrero I, et al. 2020. J Clin Med. 9:959.
  10. Alvarez-Lafuente R, et al. 2021. Front Immunol. 12:778204.
Gene ID
15976 View all products for this Gene ID
UniProt
View information about IFNAR2 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 02/28/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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