Recombinant Mouse CXCL12 (SDF-1β) (carrier-free)

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Other Names
Pre-B-cell growth-stimulating factor (PBSF), stromal cell derived factor 1 isoform beta precursor (SDF-1β)
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CXCL12B_Mouse_Recombinant_Protien_BA_070513
THP-1 cells chemoattracted by mouse CXCL12 (SDF-1β).
  • CXCL12B_Mouse_Recombinant_Protien_BA_070513
    THP-1 cells chemoattracted by mouse CXCL12 (SDF-1β).
Cat # Size Price Quantity Avail. Save
589802 10 µg 148€
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589804 25 µg 260€
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589806 100 µg 468€
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Description

CXCL12 is commonly known as SDF-1, with two variants, SDF-1α (89 amino acids) and SDF-1β (93 amino acids) derived from alternative splicing. They have identical amino acid sequences, differing only by the addition of four amino acids at the carboxy-terminus of the β form. CXCL12β is constitutively expressed by stromal cells. CXCL12α and β are ubiquitously expressed, and it has been suggested that their activity is regulated through differential proteolytic processing. Proteases present in serum process CXCL12α initially at the carboxy-terminus (through carboxypeptidase N) and subsubsequently at the amino-terminus (through CD26); while CXCL12β is only processed at the amino-terminus. These proteolytic processes affect the chemokine's ability to bind to heparin and cells as well as its ability to stimulate proliferation and chemotaxis. CXCL12α is inactivated by other enzymes such as metalloproteases, serine proteases, and leukocyte elastase. CXCL12/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. Also, CXCL12 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. CXCL12 promotes survival by inducing anti-apoptotic proteins, decreasing apoptotic proteins, and increasing transcription of cell survival genes. Data suggests that CXCL12β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse CXCL12 (SDF-1β) amino acids Lys 22-Met 93 (Accession# NP_038683.1) was expressed in E. coli.
Molecular Mass
The 72 amino acid recombinant protein has a predicted molecular mass of approximately 8.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 11 and 14 kD by SDS-PAGE respectively. The N-terminal amino acid is Lysine.
Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its ability to chemoattract THP-1 cells in a dose dependent manner.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Antigen Details

Structure
Chemokine
Distribution

Expressed by bone marrow stromal cells, endothelial cells, epithelial cells, pericytes, dendritic cells, fibroblasts

Function
CXCL12 is a constitutive chemokine involved in lung, brain, joint, and intestine inflammation. CXCL12 is chemotactic for T, B, and cancer cells. CXCL12/CXCR4 pair plays a role in mobilization of CD34+ progenitors to peripheral blood. CXCL12 is important in morphogenesis, angiogenesis, and cancer metastasis.
Interaction
Endothelial cells, epithelial cells, T and B cells, monocytes
Ligand/Receptor
CXCR4
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, Cytokines/Chemokines
Antigen References

1. Nagasawa T, et al. 1994. Proc. Natl. Acad. Sci. USA 91:2305.
2. Muller A, et al. 2001. Nature 410:50.
3. Valenzuela-Fernandez A, et al. 2002. J. Biol. Chem. 277:15677.
4. De La Luz Sierra M, et al. 2004. Blood 103:2452.
5. Kortesidis A, et al. 2005. Blood 105:3793.
6. Davis DA, et al. 2005. Blood 105:4561.
7. Zlotnik A. 2008. J. Pathol. 215:211.
8. Herberg S, et al. 2013 PLoS One 8:e58207.

Gene ID
6387 View all products for this Gene ID
UniProt
View information about CXCL12 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 2    Revision Date: 06/24/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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