Purified anti-Keratin 14 Antibody

Pricing & Availability
Poly19053 (See other available formats)
Regulatory Status
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Rabbit Polyclonal
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Product Citations
Western blot analysis of cell lysates from HaCaT (Positive control) and U87-MG (Low expression negative control) cells using Keratin 14 Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (#406401). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 1_Poly19053_PURE_Keratin14_Antibody_1_WB_102317
    Western blot analysis of cell lysates from HaCaT (Positive control) and U87-MG (Low expression negative control) cells using Keratin 14 Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (#406401). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 2_Poly19053_PURE_Keratin14_Antibody_1_IHC_052716
    IHC staining of anti-Keratin 14 antibody (Poly19053) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 3_Poly19053_PURE_Keratin14_Antibody_2_WB_052716
    Western blot of anti-Keratin 14 antibody (Poly19053). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml, overnight at 4°C, followed by incubation with a fluorescent labeled goat anti-mouse secondary antibody.
  • KifLiakath
    Whole mount mouse tail epidermis was stained with purified anti-Keratin 14 (green, clone Poly19053), purified anti-Keratin 15 (orange, clone Poly18339) and DAPI (blue). Image generously submitted to the 2017 Cell Life Imaging Competition by Kif Liakath.
  • Poly19053_PURE_Keratin-14_Antibody_ICC_112817
    Immunofluorescence of HaCaT cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Keratin 14 primary Antibody (Clone Poly 19053). 2 µg/ml (1:250 dilution) Alexa Fluor® 594 (red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Anti-Keratin 14 primary antibody concentrations for (A, B) is 2 µg/ml (1:250 dilution), (C) is 1 µg/ml (1:1000 dilution), (D) is 0.5 µg/ml (1:1000 dilution).
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905303 25 µg 67€
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905304 100 µg 216€
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Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

IHC-P - Quality tested
ICC, WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 2 ug/ml (1:250-1:1000) is suggested. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hu Y, et al. 2001. Nature 410:710. PubMed
  2. Yuspa SH, et al. 1989. J. Cell. Biol. 109:1207. PubMed
Product Citations
  1. Jones KB et al. 2018. Cell stem cell. 24(1):183-192 . PubMed
  2. Giraddi RR et al. 2018. Cell reports. 24(6):1653-1666 . PubMed
  3. Moehring F et al. 2018. eLife. 7 pii: e31684. PubMed
  4. Nishiguchi MA et al. 2018. Cell reports. 24(13):3383-3392 . PubMed
  5. Cowie AM et al. 2018. The Journal of Neuroscience. 38(25):5807-5825 . PubMed
  6. Changarathil G, et al. 2019. PLoS One. 14:e0215908. PubMed
  7. Adams CR, et al. 2019. Elife. 8. PubMed
  8. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  9. Anderton H, et al. 2018. Cell Death Differ. 26:877. PubMed
  10. Anderton H, et al. 2017. J Invest Dermatol. 137:2371. PubMed
  11. Koenig U, et al. 2019. Autophagy. :1. PubMed
  12. Altmeier S, et al. 2016. PLoS Pathog. 12: 1005882. PubMed
  13. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  14. Abbasi S, et al. 2020. Cell Stem Cell. 27(3):396-412.e6. PubMed
  15. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
AB_2734678 (BioLegend Cat. No. 905303)
AB_2616896 (BioLegend Cat. No. 905304)

Antigen Details

Keratin 14 is a 472 amino acid protein with a molecular mass of 52 kD.

Tissue distribution: skin.
Cellular distribution: extracellular, nucleus, cytoskeleton, and cytosol.

Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Wend P, et al. 2013. EMBO J. 32(14):1977-89. PubMed
2. Welm AL, et al. 2005. Proc. Natl. Acad. Sci. USA 102(12):4324. (IHC) PubMed
3. Liang Y. 2011. Pathalog. Res. Int. 2011:93674. (IHC) PubMed

Gene ID
3861 View all products for this Gene ID
View information about Keratin 14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 08/12/2022

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Keratin 14 Polyclonal Antibody, Purified

  • Purified anti-Keratin 14


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