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Purified anti-JMJD6 Antibody

Pricing & Availability
Clone
W20240B (See other available formats)
Regulatory Status
RUO
Other Names
Bifunctional arginine demethylase and lysyl hydroxylase JMJD6, Jumonji domain-containing protein 6, phosphatidylserine receptor (PSR, PTDSR1)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
a. W20240B_-PURE_JMJD6_-IHC-P_Antibody_071423
IHC staining of purified anti-JMJD6 (clone W20240B) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • a. W20240B_-PURE_JMJD6_-IHC-P_Antibody_071423
    IHC staining of purified anti-JMJD6 (clone W20240B) on formalin-fixed paraffin-embedded human testis tissue. Following antigen retrieval using Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) or with (panel B) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
  • b. W20240B_-PURE_JMJD6_-ICC_071423
    A549 cells were fixed with 4% Fixation Buffer (Cat. No. 420801) for 10 minutes, permeabilized with 100% ice-cold methanol for 10 minutes, and blocked with 5% FBS for 1 hour at room temperature. Cells were then stained with 1.25 µg/mL of purified anti-JMJD6 (clone W20240B), followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801, panel A). Images were captured with a 60X objective. Scale bar: 50 µm
  • c. W20240B_-PURE_JMJD6_-WB_071423
    Whole cell extracts (15 µg total protein) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-JMJD6 (clone W20240B) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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615551 25 µg 92€
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615552 100 µg 316€
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Description

Bifunctional arginine demethylase and lysyl hydroxylase, also known as Jumonji domain-containing protein 6 (JMJD6), is a multifunctional enzyme that plays a critical role in regulating transcription and translation. One of its primary roles is the regulation of RNA splicing, where it acts as a co-factor for RNA-binding proteins that modulate alternative splicing events. Additionally, JMJD6 can also directly modify mRNA, modulating its stability, processing and translation. Previously, JMJD6 had been proposed to act as a histone demethylase; however, this function is a matter of some dispute. 

JMJD6 has been shown to regulate immune responses, cell differentiation, and embryogenesis. Overall, JMJD6 plays an important role in coordinating different cellular pathways, and its dysregulation has been linked to various human diseases, including cancer and autoimmune disorders.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein corresponding to the N-terminus of human JMJD6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product only weakly recognizes mouse JMJD6 in Western Blot and is not recommended for that purpose

For ICC, fixation/permeabilization with 4% PFA followed by 100% ice-cold methanol is recommended. Fixation/permeabilization with 10% ice-cold methanol or 4% PFA followed by 0.5% Triton-X is not recommended.

RRID
AB_3068229 (BioLegend Cat. No. 615551)
AB_3068229 (BioLegend Cat. No. 615552)

Antigen Details

Structure
JMJD6 is a 403 amino acid protein with a predicted molecular weight of 46.5 kD
Distribution

JMJD6 is found primarily in the nucleus

Function
Arginine demethylase, lysine hydroxylase
Ligand/Receptor
Originally identified as a receptor for phosphatidyl serine, this is no longer thought to be relevant to JMJD6 function
Biology Area
Angiogenesis, Chromatin Remodeling/Epigenetics
Molecular Family
Enzymes and Regulators
Antigen References
  1. Hahn P, et al. 2010. PLoS One. 5:e13769.
  2. Kwok J, et al. 2017. Front Genet. 8:32. 
  3. Bottger A, et al. 2015. Biochem J. 468:191-202.
  4. Wang K, et al. 2022. Front Immunol. 13:859893.
Gene ID
23210 View all products for this Gene ID
UniProt
View information about JMJD6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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