Purified anti-human IL-34 Antibody

Pricing & Availability
Clone
E0320E7 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin 34, C16orf77
Isotype
Mouse IgG2b, λ
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Product Citations
publications
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361301 50 µg 100€
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Description

Human IL-34 shares a sequence identity of 99.6%, 72% and 71% with chimpanzee, rat, and mouse IL-34, respectively, on the amino acid level. The IL-34 gene is syntenic in the human, chimpanzee, rat, and mouse genomes. IL-34 has no apparent consensus structural domain or motif, and does not share sequence homology with M-CSF; nevertheless, it binds to the CSFR. These two cytokines are not identical in biological activity and signal activation. IL-34 and CSF show an equivalent ability to support cell growth or survival. However, these cytokines are differing in their ability to induce the production of chemokines (MCP-1 and eotaxin-2) in primary macrophages, the morphological change in TF-1-fms cells, and the migration of J774A.1 cells. The use of monoclonal antibodies against the CSFR suggests differential domain binding of the receptor to IL-34 and CSF and as a result, different bioactivities and signal activation kinetics/strength are produced for these cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-34
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of human IL-34 recombinant protein ranging from 5000 to 78 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture: The purified E0320E7 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated E033B8 antibody (Cat. No. 361401) as the detection antibody and recombinant human IL-34 (Cat. No. 577909) as the standard.

Note: For testing human IL-34 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human IL-34 ELISA Kit with Pre-coated Plates (Cat. No. 439607 & 439608) are specially developed and recommended.

Product Citations
  1. Han N, et al. 2021. STAR Protocols. 2(2):100460. PubMed
RRID
AB_2563032 (BioLegend Cat. No. 361301)

Antigen Details

Structure
Secreted glycoprotein, homodimer
Distribution
IL-34 mRNA is expressed in many different tissues including: spleen, heart, brain, lung, liver, kidney, thymus, testes, ovary, small intestine, prostate, and colon
Function
IL-34 increases monocyte viability, induces macrophage proliferation, and synergizes with other cytokines to generate macrophages and osteoclasts from cultured progenitors. IL-34 promotes the formation of the colony forming unit-macrophage (CFU-M) in human bone marrow culture. IL-34 supports RANKL-induced osteoclastogenesis by promoting the adhesion and proliferation of osteoclast progenitors. Human IL-34 promotes osteoclast differentiation from peripheral blood mononucleated cells. In addition, systematic administration of IL-34 to mouse increases the number of CD11b+ cells and reduces bone mass. IL-1ß and TNF-α induce the expression of interleukin-34 mRNA in osteoblasts. IL-34 is also induced in macrophages infected with equine infectious anemia virus (EIAV).
Interaction
Macrophages, monocytes, myeloid cells
Ligand/Receptor
CSF-1R
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Clanchy FI, et al. 2006. J. Leuko. Biol. 79:757.
2. Lin H, et al. 2008. Science 320:807.
3. Chihara T, et al. 2010. Cell. Death Differ. 17:1917.
4. Wei S, et al. 2010. J. Leuko. Biol. 88:495.
5. Eda H, et al. 2011. Rheumatol. Int. 31:1525.

Gene ID
146433 View all products for this Gene ID
UniProt
View information about IL-34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/23/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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  • Purified anti-human IL-34

    IL34_Human_ELISA_011614
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