PE anti-mouse TCR Vβ5.1, 5.2 Antibody

Pricing & Availability
MR9-4 (See other available formats)
Regulatory Status
Other Names
Vβ5 T-cell receptor, T cell receptor beta 5
Mouse IgG1, κ
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Product Citations
C57BL/6 splenocytes stained with CD3e (145-2C11) APC and MR9-4 PE
  • MR9-4_PE_1_062410
    C57BL/6 splenocytes stained with CD3e (145-2C11) APC and MR9-4 PE
  • MR9-4_PE_2_062410
    C57BL/6 splenocytes stained with CD3e (145-2C11) APC and mouse IgG1 PE isotype control
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139503 25 µg 99€
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139504 100 µg 239€
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Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Murine T cell hybridoma 2HB51.8
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
  2. Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
  3. Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
  4. Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
Product Citations
  1. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  2. Takeda Y, et al. 2018. Innate Immun. 24:323. PubMed
  3. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  4. Prasad M, et al. 2021. Front Immunol. 12:644483. PubMed
  5. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  6. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  7. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  8. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  9. Wang X, et al. 2022. Elife. 11:. PubMed
AB_10612761 (BioLegend Cat. No. 139503)
AB_10613279 (BioLegend Cat. No. 139504)

Antigen Details

Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.

Expressed on a subset of TCR αβ+ T cells.

Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
MHC-Peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
Antigen References

1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171. 
2. Sim GK and Augustin AA. 1985. Cell 42:89. 
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114.

Gene ID
21577 View all products for this Gene ID
View information about TCR Vbeta5.1 5.2 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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