PE anti-mouse Ly6K Antibody

Pricing & Availability
Clone
MK34 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6K, Ly-6K, mLy-6K
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MK34_PE_Ly6K_Antibody_042816
Ly6K transfected-HEK-293 cells were stained with clone MK34 (filled histogram) PE or rat IgG2a, κ PE isotype control (open histogram).
  • MK34_PE_Ly6K_Antibody_042816
    Ly6K transfected-HEK-293 cells were stained with clone MK34 (filled histogram) PE or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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151304 100 µg 220€
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Description

Lymphocyte antigen 6K (Ly6K) is a 17 kD GPI-anchored membrane protein expressed by testicular germ cells. Ly6K associates with TEX101 and may have a role in spermatogenesis. It is also expressed in some cancer cell types; a recent study also demonstrated its expression in plasma cells. A soluble 12 kD form of Ly6K has been reported.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse testicular homogenate.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections1, western blotting1, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Maruyama M, et al. 2010. Biochem. Biophys. Res. Commun. 402:75. (IHC-F, IP, WB)
RRID
AB_2616815 (BioLegend Cat. No. 151304)

Antigen Details

Structure
GPI-anchored membrane protein of 17 kD, soluble protein of 12 kD.
Distribution

Testicular germ cells, some cancer cell types, and plasma cells.

Function
Possible role in spermatogenesis.
Interaction
TEX101.
Cell Type
B cells, Plasma cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Antigen References

1. Fujihara Y, et al. 2014. Biol. Reprod. 90:60.
2. von Allmen CE, et al. 2008. Mol. Immunol. 45:2727.
3. Yoshitake H, et al. 2008. Biochem. Biophys. Res. Commun. 372:277.
4. de Nooij-van Dalen AG, et al. 2003. Int. J. Cancer 103:768.

Gene ID
76486 View all products for this Gene ID
UniProt
View information about Ly6K on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/28/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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