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PE anti-mouse Allergin-1 Antibody

Pricing & Availability
Clone
TX83 (See other available formats)
Regulatory Status
RUO
Other Names
Allergy inhibitory receptor 1, mast cell Ag-32, MCA-32
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TX-83_PE_1_072611
C57BL/6 mouse peritoneal cavity cells were stained with c-Kit PerCP, FcεR1α APC and Allergin-1 (clone TX83) PE (top), or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the c-Kit+ population.
  • TX-83_PE_1_072611
    C57BL/6 mouse peritoneal cavity cells were stained with c-Kit PerCP, FcεR1α APC and Allergin-1 (clone TX83) PE (top), or mouse IgG1, κ PE isotype control (bottom). Data shown was gated on the c-Kit+ population.
  • TX-83_PE_2_072611
See PE spectral data
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141805 25 µg 108€
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Description

Allergin-1, also known as mast cell Ag-32, is a 60 kD single pass transmembrane protein. It contains one Ig-like C2-type (immunoglobulin-like) domain in the extracellular region and one immunoreceptor tyrosine-based inhibitory motif (ITIM)-like domain in the cytoplasmic region. Allergin-1 is expressed on mast cells, dendritic cells, and macrophages as well as at low levels on neutrophils. After tyrosine phosphorylation, allergin-1 recruits PTPN6, PTPN11, and INPP5D, which results in a diminution of IgE-mediated degranulation in mast cells. No ligands have been identified for this molecule.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Transfected cells expressing allergin-1-Flag
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Inhibits FcεRI-mediated degranulation in bone marrow-derived cultured mast cells1. Additional reported applications (for the relevant formats) include: blocking1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hitomi K, et al. 2010. Nat. Immunol. 11:601. (Block)
RRID
AB_10900438 (BioLegend Cat. No. 141805)

Antigen Details

Structure
Single pass transmembrane protein of 60 kD; contains one Ig-like C2-like domain and one ITIM-like domain
Distribution
Mast cells, dendritic cells, macrophages, neutrophils
Function
Inhibits IgE-mediated degranulation of mast cells
Interaction
PTPN6, PTPN11 and INPP5D
Cell Type
Mast cells, Dendritic cells, Macrophages, Neutrophils
Biology Area
Cell Biology, Immunology, Signal Transduction
Antigen References

1. Hitomi K, et al. 2010. Nat. Immunol. 11:601.
2. Pirozzi G, et al. 1995. J. Immunol. 155:5811.

Gene ID
380732 View all products for this Gene ID
UniProt
View information about Allergin-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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