Loading...

Pacific Blue™ anti-human FcεRIα Antibody

Pricing & Availability
Clone
AER-37 (CRA-1) (See other available formats)
Regulatory Status
RUO
Other Names
FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
AER-37_PB_1_052610
Human peripheral leukocytes stained with CD203c (NP4D6) PE and AER-37 Pacific Blue™ (top) or mouse IgG2b, κ Pacific Blue™ isotype control(bottom) (gated on lymphocyte population)
  • AER-37_PB_1_052610
    Human peripheral leukocytes stained with CD203c (NP4D6) PE and AER-37 Pacific Blue™ (top) or mouse IgG2b, κ Pacific Blue™ isotype control(bottom) (gated on lymphocyte population)
  • AER-37_PB_2_052610
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
334618 100 tests 315€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4

Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
  2. Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
RRID
AB_10575628 (BioLegend Cat. No. 334618)

Antigen Details

Structure
Ig superfamily, 50 kD
Distribution

Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients

Function
Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor
IgE
Cell Type
Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Fc Receptors
Antigen References

1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.

Gene ID
2205 View all products for this Gene ID
UniProt
View information about FcepsilonRIalpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human FcεRIα

  • Biotin anti-human FcεRIα

  • FITC anti-human FcεRIα

  • PE anti-human FcεRIα

  • Alexa Fluor® 647 anti-human FcεRIα

  • PerCP anti-human FcεRIα

  • APC anti-human FcεRIα

  • Pacific Blue™ anti-human FcεRIα

  • PE/Cyanine7 anti-human FcεRIα

  • PerCP/Cyanine5.5 anti-human FcεRIα

  • Brilliant Violet 421™ anti-human FcεRIα

  • Brilliant Violet 510™ anti-human FcεRIα

  • Brilliant Violet 605™ anti-human FcεRIα

  • APC/Cyanine7 anti-human FcεRIα

  • Alexa Fluor® 700 anti-human FcεRIα

  • PE/Dazzle™ 594 anti-human FcεRIα

  • Brilliant Violet 711™ anti-human FcεRIα

  • Alexa Fluor® 488 anti-human FcεRIα

  • TotalSeq™-A0352 anti-human FcεRIα

  • TotalSeq™-C0352 anti-human FcεRIα

  • APC/Fire™ 750 anti-human FcεRIα

  • Ultra-LEAF™ Purified anti-human FcεRIα

  • TotalSeq™-B0352 anti-human FcεRIα

  • TotalSeq™-D0352 anti-human FcεRIα

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account