Pacific Blue™ anti-human CD182 (CXCR2) Antibody

Pricing & Availability
5E8/CXCR2 (See other available formats)
Regulatory Status
Other Names
CD182, CXCR2, CD128b, IL-8RB, Type II IL-8 receptor
Mouse IgG1, κ
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Product Citations
Human peripheral blood granulocytes were stained with CD182 (clone 5E8/CXCR2) Pacific Blue™ (filled histogram) or Mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
  • 5E8slashCXCR2_PB_CD182_Antibody_081618
    Human peripheral blood granulocytes were stained with CD182 (clone 5E8/CXCR2) Pacific Blue™ (filled histogram) or Mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
320723 25 tests 104€
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320724 100 tests 221€
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CXCR2 is a 67-70 kD seven-transmembrane protein, also known as IL-8 receptor B (IL-8RB), CD182, and CD128b. It is a CXC chemokine receptor belongs to G protein-coupled receptor (GPCR) family. CXCR2 is expressed as homodimer or heterodimer with CXCR1 and found on granulocytes, NK cells, subset of T lymphocytes, mast cells, monocytes, endothelial cells, megakarocytes, and oligodendrocytes. CXCR2 mediates neutrophil activation and chemotaxis, megakaryocytic proliferation, and angiogenesis via binding its ligands including IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), and GRO-α,β,γ (CXCL1, CXCL2, CXCL3).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human CXCR2 transfected L1.2 cells
Pacific Blue anti-human CD182 CXCR2 Antibody
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The 5E8/CXCR2 antibody is useful for immunofluorescent staining and flow cytometric analysis of CXCR2 expression.

Application References

(PubMed link indicates BioLegend citation)
  1. Kyriakakis E, et al. 2011. J Leukoc Biol. 90:929. PubMed.
Product Citations
  1. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
AB_2750216 (BioLegend Cat. No. 320723)
AB_2800843 (BioLegend Cat. No. 320724)

Antigen Details

Seven transmembrane spanning, G protein-coupled receptor (CPCR), 67-70 kD, homodier or teterodimer with CXCR1

Granulocytes, NK cells, subset of T cells, monocytes, endothelial cells, oligodendrocytes

Neutrophil activation, chemotaxis, angiogenesis
IL-8(CXCL8), NAP-2(CXCL7), GCP-2(CXCL6), GRO-& alpha;,ß, γ (CXCL1, CXCL2, CXCL3)
Cell Type
Granulocytes, NK cells, T cells, Monocytes, Endothelial cells, Oligodendrocytes, Neutrophils
Biology Area
Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Chuntharapai A, et al. 1994. J. Immunol. 153:5682.
2. Wilson S, et al. 2005. J. Biol. Chem. 280:28663.
3. Emadi S, et al. 2005. Blood 105:464.
4. Omari KM, et al. 2005. Brain 128:1003.
5. Juremalm M and Nilsson G. 2005. Chem. Immunol. Allergy 87:130.
6. Wolf M, et al. 1998. Eur. J. Immunol. 28:164.

Gene ID
3579 View all products for this Gene ID
View information about CD182 on

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 08/16/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD182 (CXCR2)

  • PE anti-human CD182 (CXCR2)

  • APC anti-human CD182 (CXCR2)

  • Alexa Fluor® 488 anti-human CD182 (CXCR2)

  • Alexa Fluor® 647 anti-human CD182 (CXCR2)

  • PE/Cyanine7 anti-human CD182 (CXCR2)

  • PerCP/Cyanine5.5 anti-human CD182 (CXCR2)

  • APC/Fire™ 750 anti-human CD182 (CXCR2)

  • PE/Dazzle™ 594 anti-human CD182 (CXCR2)

  • Pacific Blue™ anti-human CD182 (CXCR2)

  • TotalSeq™-A1273 anti-human CD182 (CXCR2)


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