LEGEND MAX™ Mouse IFN-α1 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
IFNa, IFN-a1, IFN-alpha, IFNalpha, Interferon alpha, Interferon alpha-1, If, Ifa1, If1ai14
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LEGEND-MAX_Mouse_IFN-alpha_100523
  • LEGEND-MAX_Mouse_IFN-alpha_100523
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447907 1 Pre-coated Plate 480€
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Description

IFN-α1 is a chemokine produced by dendritic cells, lymphocytes, macrophages and other cells in response to infection. It is expressed in the thymus, placenta, and the spleen, and is produced primarily by hematopoietic cells, in particular plasmacytoid dendritic cells found in the peripheral lymphoid organs. IFN-α1 binds to IFNAR, causing a cascade that promotes expression of IFN stimulated genes, which have anti-viral, anti-tumor, anti-proliferative, and immunomodulatory functions.

The BioLegend LEGEND MAX™ Mouse IFN-α1 ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is precoated with a mouse monoclonal anti-mouse IFN-α1 antibody. The detection antibody is a biotinylated polyclonal anti-mouse IFN-α1 antibody. This kit is specifically designed for the accurate quantitation of mouse IFN-α1 in cell culture supernatant, serum and plasma. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IFN-α1 Pre-coated 96-well Strip Microplate
  • Mouse IFN-α1 Detection Antibody
  • Mouse IFN-α1 Lyophilized Standard
  • Avidin HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution D
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA

Sensitivity
1.67 pg/mL
Standard Range
7.81 - 500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 μL to 1,000 μL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Type
Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
NA
UniProt
View information about IFN-alpha1 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 10/05/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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