FITC anti-mouse CD9 Antibody

Pricing & Availability
Clone
MZ3 (See other available formats)
Regulatory Status
RUO
Other Names
DRAP-27, MRP-1, p-24
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MZ3_FITC_011409.jpg
C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 FITC
  • MZ3_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with RB6-8C5 (Gr-1) PerCP/Cy5.5 and MZ3 FITC
See FITC spectral data
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124807 25 µg 32,00€
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124808 100 µg 88,00€
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Description

CD9 is a surface glycoprotein of the tetraspannin family. It is expressed on a variety of cells, including nerve, muscle cells and many cells of hematopoietic origin. CD9 is found to participate in forming a large molecular cell complex with other membrane proteins, such as MHC class II, CD19, CD5 and other TM4SF molecules. It is reported that CD9 is a marker of marginal zone B cells, B1 cells and plasma cells. The diverse functions of CD9 may largely depend upon its associated molecules on different cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Won WJ, et al. 2002. J. Immunol. 168:5605. (FC, WB)
Product Citations
  1. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  2. Kırbaş OK, et al. 2019. Sci Rep. 9:19159. PubMed
  3. Hu Q, et al. 2018. Nat Biomed Eng. 0.660416667. PubMed
  4. Inoue N, et al. 2015. Nat Commun. 6: 8858. PubMed
  5. Inoue N, et al. 2021. eLife. 10:00. PubMed
  6. Shao M, et al. 2021. Cell Stem Cell. 28(4):685-701.e7. PubMed
  7. Joffin N, et al. 2021. Cell Stem Cell. 28(4):702-717.e8. PubMed
  8. Shan B, et al. 2020. Nat Metab. 1332:2. PubMed
  9. de Winde CM, et al. 2021. J Cell Sci. 134: . PubMed
RRID
AB_1279324 (BioLegend Cat. No. 124807)
AB_1279321 (BioLegend Cat. No. 124808)

Antigen Details

Structure
24 kd surface glycoprotein belonging to the tetraspanin (TM4SF)family which is characterized by four transmembrane-spanning domains and two extracellular domains.
Distribution

CD9 is expressed in nerve, muscle, keratinocytes, fibroblasts and a variety of hematopoietic cells including monocytes, macrophages, granulocytes , platelets and activated T and B cells.

Interaction
CD9 may participate in forming large molecular complexes with other associated molecules.
Cell Type
B cells, Embryonic Stem Cells, Fibroblasts, Granulocytes, Macrophages, Monocytes, Platelets, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Boucheix C, et al. 1991. J. Biol. Chem. 266:117
2. Lanza F, et al. 1991. J. Biol. Chem. 266:10638

Gene ID
12527 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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