- TRFK4 (See other available formats)
- Other Names
- Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T-cell replacing factor-1 (TRF-1)
- Rat IgG2a, κ
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- Product Citations
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IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. The native protein can have variable molecular weight as a result of glycosylation. Monomeric mouse IL-5 is a 113 amino acid protein with a reported molecular weight of 35-37 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors and stimulates the activation, and differentiation of eosinophils, and promotes the generation of cytotoxic lymphocytes . Mouse IL-5 induces the differentiation and proliferation of pre-activated B-cells and stimulates the production and secretion of IgM and IgA by B-cells stimulated with bacterial endotoxin. The TRFK4 antibody reacts with mouse interleukin-5 (IL-5). The TRFK4 antibody can neutralize the bioactivity of natural or recombinant IL-5.Product Details
- Antibody Type
- Host Species
- Partially-purified T cell clone supernatant
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-5 recombinant protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA1-4 or ELISPOT5-7 Detection: The biotinylated TRFK4 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified TRFK5 antibody (Cat. No. 504302/504308) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1 of mouse IL-5 bioactivity. The LEAF™ purified TRFK5 antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of mouse and human IL-5 bioactivity (Cat. No. 504308).
Note: For testing mouse IL-5 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431201 to 431206) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Schumacher, J., et al. 1988. J. Immunol. 141:1576.
- Abrams, J., et al. 1992. Immunol. Rev. 127:5.
- Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
- Sander, B., et al. 1993. J. Immunol. Meth. 166:201.
- Taguchi, T., et al. 1990. J. Immunol. Meth. 128:65.
- Taguchi, T., et al. 1990. J. Immunol. 145:68.
- Klinman, D., et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19.
AB_2296156 (BioLegend Cat. No. 504401)
AB_2127162 (BioLegend Cat. No. 504402)
- Cytokine; dimer; 40-50 kD (Mammalian)
- Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
- Cell Sources
- Mast cells, T cells, eosinophils
- Cell Targets
- Eosinophils, B cells
- Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immunol. 35:593.
- Gene ID
- 16191 View all products for this Gene ID
- View information about IL-5 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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