Loading...

Biotin anti-mouse CD103 Antibody

Pricing & Availability
Clone
2E7 (See other available formats)
Regulatory Status
RUO
Other Names
Integrin αIEL chain, Integrin αE chain, αE integrin, ITGAE
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
2E7_Biotin_021808
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated 2E7, followed by Sav-PE
  • 2E7_Biotin_021808
    C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated 2E7, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
121403 50 µg 72€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
121404 200 µg 165€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to the integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse intestinal intraepithelial lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,7 of acetone-fixed frozen sections, immunofluorescence2, and in vitro activation1.

Application References

(PubMed link indicates BioLegend citation)
  1. LeFrancois L, et. al, 1994. Eur. J. Immunol. 24:635. (FC, IHC, IP)
  2. Mysorekar IU, et. al, 2002. J. Biol. Chem. 277:37811. (FC, IF)
  3. Mikami N, et al. 2011. J. Immunol. 186:6886. PubMed
  4. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  5. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  6. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  7. Xiao B, et al. 2015. PLoS One 1:e0115333. (IHC)
Product Citations
  1. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  2. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  3. Murphy S, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:871. PubMed
  4. Takano T, et al. 2020. Front Immunol. 11:1555. PubMed
  5. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  6. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
RRID
AB_535946 (BioLegend Cat. No. 121403)
AB_535947 (BioLegend Cat. No. 121404)

Antigen Details

Structure
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution

Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.

Function
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand/Receptor
E-Cadherin
Cell Type
Dendritic cells, Lymphocytes, T cells, Tregs
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201.
2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852.
3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635.
4. Sung SS, et al. 2006. J. Immunol. 176:2161.
5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063.
6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.

Gene ID
16407 View all products for this Gene ID
UniProt
View information about CD103 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 05/04/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD103

  • Biotin anti-mouse CD103

  • PE anti-mouse CD103

  • Alexa Fluor® 488 anti-mouse CD103

  • Alexa Fluor® 647 anti-mouse CD103

  • Alexa Fluor® 594 anti-mouse CD103

  • Brilliant Violet 510™ anti-mouse CD103

  • APC anti-mouse CD103

  • PerCP/Cyanine5.5 anti-mouse CD103

  • Pacific Blue™ anti-mouse CD103

  • FITC anti-mouse CD103

  • Brilliant Violet 421™ anti-mouse CD103

  • PE/Cyanine7 anti-mouse CD103

  • APC/Cyanine7 anti-mouse CD103

  • PE/Dazzle™ 594 anti-mouse CD103

  • Brilliant Violet 605™ anti-mouse CD103

  • Brilliant Violet 711™ anti-mouse CD103

  • TotalSeq™-A0201 anti-mouse CD103

  • Brilliant Violet 785™ anti-mouse CD103

  • Alexa Fluor® 700 anti-mouse CD103

  • TotalSeq™-C0201 anti-mouse CD103

  • TotalSeq™-B0201 anti-mouse CD103

  • PE/Cyanine5 anti-mouse CD103
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account