APC anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_APC_122107
C57BL/6 bone marrow cells stained with TER-119 APC and anti-mouse CD45 FITC
  • TER-119_APC_122107
    C57BL/6 bone marrow cells stained with TER-119 APC and anti-mouse CD45 FITC
See APC spectral data
Cat # Size Price Quantity Avail. Save
116211 25 µg 32€
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116212 100 µg 100€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Koury D, et al. 2017. Int J Parasitol. 10.1016/j.ijpara.2017.05.009. PubMed
  2. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  3. Lam J, et al. 2018. Nat Commun. 9:2418. PubMed
  4. Wang L, et al. 2018. Bone Res. 6:22. PubMed
  5. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  6. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  7. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  8. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
  9. Wang H, et al. 2019. J Clin Invest. 129:598. PubMed
  10. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  11. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  12. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  13. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
  14. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  15. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  16. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
  17. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
  18. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  19. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  20. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  21. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
  22. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  23. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  24. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
  25. Li G, et al. 2020. J Clin Invest. 130:1233. PubMed
  26. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
  27. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  28. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  29. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
  30. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  31. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  32. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
  33. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
  34. Bilodeau M, et al. 2009. PLoS One. 4:e7500. PubMed
  35. Guihard S, et al. 2010. Blood. 115:3686. PubMed
  36. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  37. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  38. Song J, et al. 2014. Exp Cell Res. 327:123. PubMed
  39. Khoriaty R, et al. 2014. Mol Cell Biol. 34:3721. PubMed
  40. Yin Y, et al. 2016. J Biol Chem. 291: 6923 - 6935. PubMed
  41. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
  42. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
  43. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
RRID
AB_313712 (BioLegend Cat. No. 116211)
AB_313713 (BioLegend Cat. No. 116212)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/04/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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