APC anti-human CD227 (MUC-1) Antibody

Pricing & Availability
16A (See other available formats)
Regulatory Status
Other Names
Mucin-1, MUC-1, Episialin, HMFG antigen, MAM6
Mouse IgG1, λ
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Product Citations
PHA-stimulated (3-days) human peripheral blood mononuclear cells were stained with CD3 FITC and CD227 (clone 16A, top) APC or mouse IgG1, κ APC isotype control (bottom).
  • 16A_APC_CD227_Antibody_FC_1_010815
    PHA-stimulated (3-days) human peripheral blood mononuclear cells were stained with CD3 FITC and CD227 (clone 16A, top) APC or mouse IgG1, κ APC isotype control (bottom).
  • 16A_APC_CD227_Antibody_FC_2_010815
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355607 25 tests 105€
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355608 100 tests 290€
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Mucin-1 (MUC-1), cell surface associated or polymorphic epithelia mucin, is a 500-1000 kD proteoglycan expressed by activated T cells, mucosal epithelial cells, and aberrantly expressed on most breast cancers.  In normal cells, CD227 is heavily glycosylated, whereas in cancerous cells, the glycosylation is incomplete and premature sialation is also observed.  The protein is anchored to the apical surface of the epithelial cell and functions as a lubricant to keep the cell hydrated and to protect against pathogens.  It can also function as a signaling molecule by forming a MUC-1/SOS1/GrB2 complex.  MUC-1 can interact with cancer antigens such as Her2/neu.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Jurkat cells transfected with MUC1
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

This clone shows stronger binding to the glycosylated compared to the non-glycosylated peptide1.

Application References

(PubMed link indicates BioLegend citation)
  1. Song W, et al. 2012. Int. J Oncol. 41:1977. PubMed
Product Citations
  1. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  2. Wang J, et al. 2022. Front Immunol. 13:863346. PubMed
  3. Norgard RJ, et al. 2021. EMBO Rep. 22:e51872. PubMed
  4. Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
AB_2565311 (BioLegend Cat. No. 355607)
AB_2565312 (BioLegend Cat. No. 355608)

Antigen Details

500-1000 kD proteoglycan (glycosylated form)

Activated T cells, mucosal epithelial cells, aberrantly expressed in most breast cancers

Complexes with SOS1 and Grb2 in RAS signaling
ICAM-1 and Her2/neu (ErbB2)
Cell Type
Dendritic cells, Epithelial cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Gendler SJ. 2001. J. Mammary Gland Biol. Neoplasia. 6:339.
2. Agrawal B, et al. 1998. Cancer Res. 58:4079.
3. Rahn JJ, et al. 2004. J. Biol. Chem. 279:29386.

Gene ID
4582 View all products for this Gene ID
View information about CD227 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/12/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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