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Alexa Fluor® 647 anti-human ACE2 Antibody

Pricing & Availability
Clone
A20069I (See other available formats)
Regulatory Status
RUO
Other Names
Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACE-2, ACEH
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A20069I_A647_ACE2_Antibody_2_122120
Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL anti-human ACE2 (clone A20069I) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A20069I_A647_ACE2_Antibody_2_122120
    Human paraffin-embedded testis tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL anti-human ACE2 (clone A20069I) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A20069I_A647_ACE2_Antibody_1_122120
    Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL anti-human ACE2 (clone A20069I) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • A20069I_A647_ACE2_Antibody_3_122120
    Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL anti-human ACE2 (clone A20069I) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data
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375803 25 µg 132€
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375804 100 µg 300€
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Description

Angiotensin-converting enzyme 2 (ACE2) is a type one transmembrane protein, which shares approximately 60% homology with ACE. The predicted human ACE2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction. Recently, ACE2 has been shown to be a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus). Based on recent single-cell RNA sequencing (scRNA-Seq) database, high ACE2 RNA expression was identified in type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, and endothelium and vascular smooth muscle cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein cover extracellular domain of ACE2 (Gln 18-Ser740)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Li T, et al. 2022. J Clin Invest. 132:. PubMed
RRID
AB_2890824 (BioLegend Cat. No. 375803)
AB_2890824 (BioLegend Cat. No. 375804)

Antigen Details

Structure
Type one transmemberance protein. Protein sequence consistent extracellular domain (Gln 18-Ser740 ) contain cytalitic domain, transmembrane domain (AA741-AA761) and cytoplasmic domain (AA762-AA805).
Distribution

Type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells,  enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, endothelium and vascular smooth muscle cells

Function
Catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction
Interaction
Angiotensin II, ADAM17, TMPRSS2
Ligand/Receptor
Functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2 (COVID-19 virus)
Cell Type
Monocytes
Biology Area
Angiogenesis, COVID-19, Immunology
Molecular Family
Enzymes and Regulators
Antigen References
  1. South AM, et al. 2020. Nat Rev Nephrol. 16:305-7.
  2. Hoffmann M, et al. 2020. Cell. 181:271-80.
  3. Sungnak W, et al. 2020. Nat Med. 26:681-7.
  4. Li W, et al. 2003. Nature. 426:450-4.
  5. Heurich A, et al. 2014. J Virol. 88:1293-30.
  6. Hamming I, et al. 2004. J Pathol. 203:631-7.
Gene ID
59272 View all products for this Gene ID
UniProt
View information about ACE2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/21/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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