BioLegend reagents work beautifully on instruments from all manufacturers and diversity of biological applications. The field of single cell, fluorescence-based multiplexing continues to grow in total multicolor capacity, sensitivity and ease of use. Cytek®’s Aurora Spectral Cytometer has a rapidly growing presence among flow cytometry research instruments. The Aurora is available with 3 or 5 laser configurations (355 nm, 405 nm, 488 nm, 561 nm and 633 nm), with the latter offering up to 64 different fluorescent parameters. This is only the theoretical limit as we continue to strive to provide fluorophores that are distinct enough to be accurately unmixed on the system. The Aurora also utilizes APD (Avalanche Photodiode) detectors which liberate the near-IR and infrared wavelengths for more efficient detection of emitted photons in this range. This page will discuss data generated in-house here at BioLegend as we test the limits of spectral flow cytometry to increase the number of fluorophores in multicolor panel development.
To view the full capabilities of spectral cytometry, read this recent publication in Cytometry A about the first 40 color flow cytometry panel.