Go-ChIP-Grade™ Purified anti-SSRP1 Antibody

Pricing & Availability
Clone
10D1 (See other available formats)
Other Names
Structure specific recognition protein 1 (SSRP1), Facilitates Chromatin Transcription 80 kD subunit, FACTp80, Recombination signal sequence recognition protein
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
10D1_GoChIP_SSRP1_Antibody_1_052118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HeLa cells (starved overnight then treated with 10% FCS) with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-SSRP1 Antibody (clone 10D1, Cat. No. 609709) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 10D1_GoChIP_SSRP1_Antibody_1_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HeLa cells (starved overnight then treated with 10% FCS) with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-SSRP1 Antibody (clone 10D1, Cat. No. 609709) or B) equal amount of Purified Mouse IgG2b, κ isotype control antibody (clone MPC-11, Cat. No. 400301) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 10D1_GoChIP_SSRP1_Antibody_2_WB_030717
    Total lysates (15 µg protein) from HeLa (lane 1) and NIH3T3 cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mLPurified anti-SSRP1 Antibody, clone 10D1 (upper) or 1:3000 diluted Purified anti-β-actin Antibody, clone Poly6221 (lower). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-SSRP1 Antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-β-actin Antibody.
  • 10D1_GoChIP_SSRP1_Antibody_3_IF_030717
    HeLa cells were stained with purified anti- SSRP-1 (10D1) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. Actin filaments were labeled in green. Nuclei were stained with DAPI (blue).
Cat # Size Price Quantity Avail. Save
609709 25 µg 75 CHF
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609710 100 µg 235 CHF
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Description

SSRP1 is a nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT complex is composed of the SUPT16H (FACT140) and the SSRP1 FACTp80 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. This protein interacts with dsDNA. The SSRP1 protein has been shown to be modified by cisplatin and may protect DNA from repair and block DNA replication. The 10D1 monoclonal antibody recognizes the human SSRP1 protein and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (partial) (308-524aa)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
IF, WB - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50-1:200 by volume.

It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

10D1 displayed a stronger affinity for SSRP1 than BioLegend clone 3E4 when tested for western blot application.

Application References

(PubMed link indicates BioLegend citation)
  1. Tan BC, et al. 2006. EMBO 25:3975. (ChIP)
  2. Carvalho S, et al. 2013. Nucleic Acids Res. 41:2881. PubMed
  3. Tan BC, et al. 2004. J. Biol. Chem. 279:9321. (Immunogen)
  4. Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed
  5. Aida M, et al. 2013. PNAS. 110:7784. PubMed
  6. Dermawan JK, et al. 2014. Mol Cancer Ther. 13:2203. PubMed
  7. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
Product Citations
  1. Kolundzic E 2018. Developmental cell. 46:611. PubMed
Publication Library
RRID
AB_2650946 (BioLegend Cat. No. 609709)
AB_2650944 (BioLegend Cat. No. 609710)

Antigen Details

Structure
SSRP family, HMG box domain; 81 kD
Distribution

Nuclear

Function
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation, binds to dsDNA. Modified by cisplatin, may protect DNA from repair and block DNA replication
Interaction
Component of FACT ATP-dependent chromatin remodeling complex, SUPT16H/Cdc68 in FACT complex, H2A/H2B dimers
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Antigen References

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.

Gene ID
6749 View all products for this Gene ID
UniProt
View information about SSRP1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03.02.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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