Go-ChIP-Grade™ Purified anti-Spt16 (FACT140 complex) Antibody

Pricing & Availability
Clone
8D2 (See other available formats)
Other Names
Facilitates Chromatin Transcription (FACT140), Spt 16 protein, Cdc68
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
1_8D2_GoChIP_Spt16_FACT140_Antibody_1_052118
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HT29 cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Spt16 (FACT140 complex) (clone 8D2, Cat. No. 607007) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ isotype control antibody (clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 1_8D2_GoChIP_Spt16_FACT140_Antibody_1_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HT29 cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Spt16 (FACT140 complex) (clone 8D2, Cat. No. 607007) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ isotype control antibody (clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR1 gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 2_8D2_GoChIP_Spt16_FACT140_Antibody_2_052118
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HT29 cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Spt16 (FACT140 complex) (clone 8D2, Cat. No. 607007) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ isotype control antibody (clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human C-FOS gene region or α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3_8D2_Spt16_Purfied_Antibody_WB_120717
    Total lysates (15 µg protein) from A549 (lane 1), Jurkat (lane 2), and Raw 264.7 cells (lane 3) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) Purified anti-Spt16 Antibody, clone 8D2 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-Spt16 Antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin Antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
  • 4_8D2_Spt16_Purified_Antibody_IF_011515
    Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL anti-Spt16 Antibody (8D2) and followed by DyLight™ 594 goat anti-mouse IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
607007 25 µg 95 CHF
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607008 100 µg 225 CHF
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Description

Spt16 is a 120-140 kD nuclear protein and a component of the FACT complex (facilitates chromatin transcription). The FACT140 complex is composed of the Spt16 and the SSRP1 proteins. This complex interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation. The 8D2 monoclonal antibody recognizes human Spt16 and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant (full length)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ChIP - Quality tested
WB, ICC - Validated 

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:50 - 1:100 by volume. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

25 µg, 100 µg of Go-ChIP-Grade™ Purified Antibody can be used for 2-4, 11-16 immunoprecipitations, respectively, at the recommended dilution. ICC positive in HeLa Cells. 

Application References

(PubMed link indicates BioLegend citation)
  1. Tan B C-M, et al. 2006. EMBO doi:10.1038/sj.emboj.7601271
  2. Adelmant G, et al. 2012. Mol Cell Proteomics. 11:411. PubMed
  3. Lolis AA, et al. 2013. J Biol Chem. 288:7676. PubMed
  4. Shakya A, et al. 2015. Mol Cell Biol. 35:1014. PubMed
RRID
AB_2721597 (BioLegend Cat. No. 607007)
AB_2721598 (BioLegend Cat. No. 607008)

Antigen Details

Structure
120-140 kD
Distribution

Nuclear

Function
Interacts with nucleosomes and histone H2A/H2B dimers to promote nucleosome disassembly and allow transcription elongation
Interaction
Component of FACT ATP-dependent chromatin remodeling complex, SSRP1 protein in FACT complex, H2A/H2B dimers
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. LeRoy G, et al. 1998. Science 282:1836.
2. Orphanides G, et al. 1998. Cell 92:105.
3. Orphanides G, et al. 1999. Nature 400:284.

Gene ID
11198 View all products for this Gene ID
UniProt
View information about Spt16 (FACT140 complex) on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12.08.2017

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