- Clone
- DO-1 (See other available formats)
- Other Names
- Tumor protein p53 (TP53)
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
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- Product Citations
- publications
-
Chromatin Immunoprecipitations (ChIP) were done with fixed and sonicated chromatin samples from 293T cells treated with tunicamycin (2 µg/mL, 8 hr). ChIP was performed with 5 µg of chromatin and either 1 µg of Go-ChIP-Grade™ purified anti-p53 antibody (Clone DO-1), or equal amount of Go-ChIP-Grade™ purified mouse IgG2a, κ isotype ctrl antibody (Clone MG2a-53, Cat. No. 401506). The enriched DNA was purified and quantified by real-time qPCR using SYBR Green and primers for the human CDKN1A promoter, and for a gene desert region on human chromosome 12. The amount of immunoprecipitated DNA in each sample is represented as a percentage of the total amount of input chromatin, which is equivalent to 100%.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 645703 | 25 µg | 95 CHF | ||||
| 645704 | 100 µg | 225 CHF | ||||
p53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation; ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- P53 N-terminal linear epitope aa 20-25
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
ChIP - Quality tested
- Recommended Usage
-
The recommended starting antibody to chromatin ratio is 1 µg of antibody and 5 µg of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent to be titrated for optimal performance before each experiment.
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Application References
(PubMed link indicates BioLegend citation) -
- Vojtesek B, et al. 1992. J Immuno Methods 151:237
- Stephen CW, et al. 1995. J Mol Biol 248:58
- RRID
-
AB_2832839 (BioLegend Cat. No. 645703)
AB_2832840 (BioLegend Cat. No. 645704)
Antigen Details
- Structure
- Tetramer; 53 kD
- Distribution
-
Nuclear when activated
- Function
- Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
- Interaction
- mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, DNA Repair/Replication, Stem Cells, Transcription Factors
- Antigen References
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1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536. - Gene ID
- 7157 View all products for this Gene ID
- UniProt
- View information about p53 on UniProt.org
Related Pages & Pathways
Pathways
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