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Direct-Blot™ HRP anti-GFAP Antibody

Pricing & Availability
Clone
2E1.E9 (See other available formats)
Regulatory Status
RUO
Other Names
Glial fibrillary acid protein
Isotype
Mouse IgG2b
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Product Citations
publications
2E1dotE9_DBHRP_GFAP_Antibody_022717
Total cell lysate from HeLa (lane 1, negative control), U87-MG (lane 2) and tissue lysate from mouse brain (lane 3) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose membrane, and probed with 1:2,500 purified anti-GFAP (clone 2E1.E9) antibody, or 1:2,500, 1:10,000 and 1:25,000 Direct-Blot™ HRP anti-GFAP (clone 2E1.E9) antibody. Purified proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. Lane M is the MW ladder.
  • 2E1dotE9_DBHRP_GFAP_Antibody_022717
    Total cell lysate from HeLa (lane 1, negative control), U87-MG (lane 2) and tissue lysate from mouse brain (lane 3) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose membrane, and probed with 1:2,500 purified anti-GFAP (clone 2E1.E9) antibody, or 1:2,500, 1:10,000 and 1:25,000 Direct-Blot™ HRP anti-GFAP (clone 2E1.E9) antibody. Purified proteins were visualized using an HRP goat anti-mouse IgG secondary antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. Lane M is the MW ladder.
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644711 100 µL 340 CHF
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Description

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Bovine spinal cord homogenate
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:2,500-1:25,000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application References

(PubMed link indicates BioLegend citation)
  1. McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221.
  2. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
RRID
AB_2650857 (BioLegend Cat. No. 644711)

Antigen Details

Structure
Around 50 kD
Distribution

Only expressed in astrocytes in the central nervous system.

Function
Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type
Astrocytes
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
2670 View all products for this Gene ID
UniProt
View information about GFAP on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03.07.2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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