Product: Alexa Fluor® 700 anti-human CD45RO |
Catalog No.: 304218 |
Joy Hsu, Specialist UCSF |
Anti-human CD45RO Antibody Alexa Fluor™ 700 | |||
Overall: | Product Quality: | Ease of Use: | |
Antibody staining for memory T cells doesn't seem to work. |
Experimental Design |
Application: | flow cytometry |
Cells used: | human tumor infiltrate |
Brief Protocol: | Cell suspension was plated in 96-well plate at 2-4 million cells/ well. Antibody was added in proper flow buffer at 1:100 for 30 minutes in the dark at 4 degrees Celsius. Used in conjunction with anti-human CD3, CD4, CD8, CD25 to parse out CD4 and CD8 T cells, activated and memory from naive T cells. |
Results Summary: | Did not see any staining, saw this repeated in multiple samples, must be an artifact of the antibody. |
Additional Notes: |
|
|
See all Reviews |
Follow Us