Product: ELISA MAX™ Deluxe Set Human TNF-a |
Catalog No.: 430205 |
Ohad Tarcic, Dr. Weizmann |
TNF-α Human (ELISA MAX™ Deluxe) | |||
Overall: | Product Quality: | Ease of Use: | |
Good TNF- alpha detection kit. |
Experimental Design |
Application: | ELISA |
Cells used: | Human mammary cells |
Brief Protocol: | 1. Coat the plates with capture antibody. 2. Block with blocking solution for 2 hours and then wash with PBST. 3. Add samples and standards to each well in triplicates for 2-4 hours in RT. 4. Add detection antibody for 1 hour and then wash with PBST. 5. Add abiding HRP for 30 minutes and then wash with PBST. 6. Add TMB and then read OD at 450nm. |
Results Summary: | TNF alpha detection. |
Additional Notes: |
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