Product: ELISA MAX™ Standard Set Mouse IL-1a |
Catalog No.: 433402 |
Dimura Rasim, Master student University of Prishtina |
Mouse IL-1α | |||
Overall: | Product Quality: | Ease of Use: | |
Cytokine production IL-1α. |
Experimental Design |
Application: | ELISA |
Cells used: | Mouse monocytes |
Brief Protocol: | Capture antibody to the plate, and 24h incubation in 4 Celsius degrees. Second day, block, add the samples and then detection antibody. After this add the enzyme and the substrate and stop with an acid. Read the plate at 450/570 nm. |
Results Summary: | Detection of IL-1alpha. |
Additional Notes: | I had a small problem with the standard, because I mixed non carefully an old one with the new one and then the standard curve was still OK. |
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