Purified anti-mouse CD45.2 Recombinant Antibody

Pricing & Availability
Clone
QA18A15 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A15_PURE_CD45dot2_RECOM_Antibody_072921.png
C57BL/6 mouse splenocytes were stained with purified anti-mouse CD45.2 recombinant (clone QA18A15) (filled histogram) or purified anti-rat IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • QA18A15_PURE_CD45dot2_RECOM_Antibody_072921.png
    C57BL/6 mouse splenocytes were stained with purified anti-mouse CD45.2 recombinant (clone QA18A15) (filled histogram) or purified anti-rat IgG1, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
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111102 100 µg 85€
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2894658 (BioLegend Cat. No. 111102)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References
  1. Basu S, et al. 2013. J Immunol Methods. 396:163-167.
  2. Xu H, et al. 2004. Stem Cells. 22:1039-1048
Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org
Go To Top Version: 1    Revision Date: 07.29.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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