PE anti-mouse Ly-6G Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
2. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
3. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
4. Sierro F, et al. 2017. Immunity. 47:374. PubMed
5. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
6. Lee T, et al. 2014. Mol Biol Cell. 25:583. PubMed
7. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
8. D'Alessandro G, et al. 2020. Eur J Immunol. 50:705. PubMed
9. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
10. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
11. Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed
12. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
13. Zhao D, et al. 2021. Innate Immun. 27:533. PubMed
14. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
15. Volmari A, et al. 2021. Hepatol Commun. 5:2104. PubMed
16. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
17. Jung J, et al. 2019. Cell Host Microbe. 25:513. PubMed
18. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
19. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
20. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
21. Cai B, et al. 2016. Proc Natl Acad Sci U S A. 113: 6526 - 6531. PubMed
22. Kozik J, et al. 2016. Sci Rep. 6:28556. PubMed
23. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
24. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
25. Gawish R, et al. 2022. Elife. 11:. PubMed
26. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
27. He J, et al. 2022. Front Immunol. 12:760138. PubMed
28. Ancey PB, et al. 2021. Cancer Res. 81:2345. PubMed
29. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
30. Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
31. Zhang Y, et al. 2018. Sci Rep. 8:5994. PubMed
32. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
33. Zhang K, et al. 2018. PLoS Pathog. 14:e1006925. PubMed
34. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
35. Putnam NE, et al. 2019. PLoS Pathog. 15:e1007744. PubMed
36. Ajendra J, et al. 2016. Sci Rep. 6:39648. PubMed
37. Bottek J, et al. 2020. Mucosal Immunol. 1.029166667. PubMed
38. Sharma K, et al. 2021. Cell Reports. 36(3):109351. PubMed
39. Hanhai Z, et al. 2021. Aging (Albany NY). 13:13108. PubMed
40. Choi YY, et al. 2021. Cell Death Dis. 12:826. PubMed
41. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
42. Ishii T,et al. 2017. Sci Rep.. 10.1038/s41598-017-12513-1. PubMed
43. Xie D, et al. 2017. PLoS One. 10.1371/journal.pone.0181454. PubMed
44. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
45. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
46. Montufar-Solis D, Klein J 2016. PLoS One. 11: 014611. PubMed
47. Sun P, et al. 2015. J Hepatol. . PubMed
48. Okada M, et al. 2014. J Biol Chem. 289:32926. PubMed
49. Muhammad W, et al. 2022. Acta Biomater. 148:258. PubMed
50. Rogic A, et al. 2021. Nat Commun. 12:6889. PubMed
51. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
52. Harding M, et al. 2014. PLoS Pathog. 10:1004379. PubMed
53. Morales D, et al. 2015. J Virol. 89:337. PubMed
54. Lin T, et al. 2015. PLoS One. 10: e0138335. PubMed
55. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
56. Yeo WS, et al. 2020. J Bacteriol. 202:00:00. PubMed
57. Webster HC, et al. 2020. J Immunol Methods. 112702:477. PubMed
58. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
59. Bae SH, et al. 2021. Theranostics. 11:2522. PubMed
60. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
61. Helm M, et al. 2022. Life (Basel). 12:. PubMed
62. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
63. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
64. Zhou Z, et al. 2019. Oxid Med Cell Longev. 2019:1209307. PubMed
65. Lemon J, et al. 2015. Infect Immun . 83: 3204-3212. PubMed
66. Mastandrea I, et al. 2022. STAR Protoc. 3:101106. PubMed
67. Ma M, et al. 2021. Front Immunol. 12:718779. PubMed
68. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
69. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
70. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
71. Galvão I, et al. 2018. Front Immunol. 9:142. PubMed
72. Metzger D, et al. 2015. J Infect Dis. 212: 445-452. PubMed
73. Winkler MJ, et al. 2020. Basic Res Cardiol. 115:67. PubMed
74. Ku MW, et al. 2020. Cell Host Microbe. 29(2):236-249.e6. PubMed
75. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
76. Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed
77. Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed
78. Zhang J, et al. 2022. iScience. 25:104963. PubMed
79. Hegde S, et al. 2020. Cancer Cell. 37(3):289-307. PubMed
80. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
81. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
82. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
83. McLaughlin PA et al. 2019. PLoS Pathog. 15(7):e1007847 . PubMed
84. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
85. Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed
86. Kojo K, et al. 2016. Sci Rep. 6:29650. PubMed
87. Soto-Díaz K, et al. 2021. Front Immunol. 12:734349. PubMed
88. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
89. Si Y, et al. 2018. J Control Release. 282:120. PubMed
90. El-Zaatari M, et al. 2014. J Immunol. 193:807. PubMed
91. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
92. Markman JL, et al. 2020. Nat Commun. 11:1613. PubMed
93. Wheeler MA, et al. 2019. Cell. 176:581. PubMed
94. Steuerman Y et al. 2018. Cell systems. 6(6):679-691 . PubMed
95. Catani J, et al. 2016. Transl Oncol. 9:565-574. PubMed
96. Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
97. Baik JE, et al. 2022. Clin Transl Med. 12:e758. PubMed
98. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
99. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
100. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
101. Umeshappa CS, et al. 2021. Cell Reports. 34(13):108919. PubMed
102. Abe M, et al. 2020. Clin Exp Immunol. 303:199. PubMed
103. Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed
104. Su M, et al. 2022. Nat Cardiovasc Res. 1:732. PubMed
105. Kim Y, et al. 2012. J Immunol. 188:4226. PubMed
106. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
107. Frenis K, et al. 2021. Basic Res Cardiol. 31:116. PubMed
108. Unudurthi SD, et al. 2020. Life Sci. 117440:247. PubMed
109. Finsterbusch M, et al. 2014. J Exp Med. 211:1307. PubMed
110. Vergadi E, et al. 2014. J Immunol. 192:394. PubMed
111. Rynkiewicz NK, et al. 2020. Sci Signal. 13:. PubMed
112. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
113. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
114. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
115. Tapia-Abellán A, et al. 2021. Sci Adv. 7:eabf4468. PubMed
116. Jiang Y, et al. 2021. Cell Death Differ. 28:3009. PubMed
117. Kimura H, et al. 2019. Sci Rep. 9:5887. PubMed
118. Hsu BE et al. 2019. Cell Rep. 27(13):3902-3915 . PubMed
119. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
120. Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed
121. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
122. Takesue S, et al. 2020. Int J Oncol. 56:596. PubMed
123. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
124. Takizawa S, et al. 2022. Mol Med. 28:91. PubMed
125. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
126. Kim M, et al. 2018. Immunity. 49:151. PubMed
127. Petkevicius K, et al. 2019. Elife. 8:e47990. PubMed
128. Baomei Wang et al. 2019. Cell reports. 26(6):1614-1626 . PubMed
129. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
130. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
131. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
132. Murray MP, et al. 2022. Cell Rep. 38:110209. PubMed
133. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
134. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
135. Penke LR, et al. 2020. Life Sci Alliance. 3:. PubMed
136. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
137. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
138. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
139. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
140. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
141. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
142. Eislmayr K, et al. 2022. Sci Adv. 8:eabj7293. PubMed
143. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
144. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
145. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
146. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
147. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
148. Vaibhav K, et al. 2020. Sci Adv. 6:eaax8847. PubMed
149. Huang W, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.115. PubMed
150. Sadhu S, et al. 2021. J Immunol. 207:1812. PubMed
151. Rao L, et al. 2020. Nat Commun. 11:4909. PubMed
152. Ding R, et al. 2022. J Neuroinflammation. 19:137. PubMed
153. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
154. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
155. Issah Y, et al. 2021. Elife. 10:. PubMed
156. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
157. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
158. Kong XF, et al. 2018. Nat Immunol. 19:973. PubMed
159. Weckbach LT, et al. 2019. J Exp Med. 216:350. PubMed
160. Kimura T, et al. 2014. Int Immunol. 26:697. PubMed
161. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
162. Hu R, et al. 2015. J Immunol. 195: 4802 - 4809. PubMed
163. Zhang T, et al. 2020. JCI Insight. 5:00. PubMed
164. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
165. Matsumoto M, et al. 2021. Cell Host Microbe. 29(6):930-940.e4. PubMed
166. Subramaniam N, et al. 2021. Blood Adv. 5:1259. PubMed
167. Li Y, et al. 2022. Theranostics. 12:5364. PubMed
168. Zhai T, et al. 2020. Clin Exp Pharmacol Physiol. 47:677. PubMed
169. Li Y, et al. 2020. Theranostics. 10:11376. PubMed
170. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
171. Moraes JA, et al. 2018. J Innate Immun. 11:330. PubMed
172. Zhao Z, et al. 2019. J Immunol Res. 2019:9561350. PubMed
173. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
174. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
175. Ishihara J, et al. 2018. Nat Commun. 9:2163. PubMed
176. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
177. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
178. Guo H, et al. 2016. J Immunol. 196: 2300 - 2308. PubMed
179. Bozoglu T, et al. 2022. Adv Sci (Weinh). 9:e2103867. PubMed
180. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
181. Wang C, et al. 2020. Mucosal Immunol. 13:22. PubMed
182. Hou Y, et al. 2013. J Nutr Biochem. 24:1555. PubMed
183. Zhang S, et al. 2016. PLoS Pathog. 12: 1005617. PubMed
184. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
185. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
186. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
187. Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
188. Wheeler MA, et al. 2020. Nature. 578:593. PubMed
189. Weng JH, et al. 2021. Nat Metab. 3:513. PubMed
190. Shin SH, et al. 2022. Clin Exp Otorhinolaryngol. . PubMed
191. Zha ZX, et al. 2022. Acta Pharmacol Sin. :. PubMed
192. Jia J, et al. 2022. Nat Commun. 13:6804. PubMed
193. Ma Y, et al. 2020. Front Immunol. 11:586685. PubMed
194. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
195. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
196. Ribechini E, et al. 2017. Blood Adv. 0.699305556. PubMed
197. Zhang B, et al. 2021. Front Neurol. 607370:12. PubMed
198. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
199. Rodgers M, et al. 2014. J Exp Med. 211:1333. PubMed
200. Hellmann J, et al. 2013. J Immunol. 191:1383. PubMed
201. Qu J, et al. 2021. Int J Biol Sci. 17:2756. PubMed

RRID

AB_1186104 (BioLegend Cat. No. 127607)
AB_1186099 (BioLegend Cat. No. 127608)

Antigen Details

Structure

A 21-35 kD GPI-anchorded membrane protein

Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type

Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID

546644 View all products for this Gene ID

UniProt

View information about Ly-6G on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Certificate of Analysis