- Regulatory Status
- RUO
- Other Names
- Multiplex assay, Flow cytometry
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price |
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The LEGENDplex™ Human TIMP Standard will be used together with the following Mix and Match Capture Beads and Human TIMP Panel Detection Antibodies to offer flexible customization within the panel (3-plex):
740482 (LEGENDplex™ Human TIMP Panel Detection Antibodies)
740374 (LEGENDplex™ Buffer Set C)
740485 (LEGENDplex™ Human TIMP-3 Capture Bead A5, 13X)
740484 (LEGENDplex™ Human TIMP-2 Capture Bead B7, 13X)
740483 (LEGENDplex™ Human TIMP-1 Capture Bead B9, 13X)
740377 or 740379 (Filter Plate or V-bottom Plate for LEGENDplex™ Assay)
The assembled Human TIMP Mix and Match Panel is a multiplex bead-based assay panel, which uses fluorescence–encoded beads suitable for use on various flow cytometers. This panel allows for the simultaneous quantification of 3 human TIMPs: TIMP-1, TIMP-2, and TIMP-3. This assay panel provides broader dynamic ranges than traditional ELISA methods, and has been validated for use with serum, plasma and cell culture supernatant samples.
Product Details
- Verified Reactivity
- Human
- Application
-
Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex
Download the LEGENDplex™ software here. - Application Notes
-
This kit detects free TIMP-1, free and complexed TIMP-2 and TIMP-3.
Antigen Details
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology
- Molecular Family
- Enzymes and Regulators
- Gene ID
- NA
- App Abbreviation (DOES NOT SHOW ON TDS):
- Multiplex - Component
Related Pages & Pathways
Pages
Related FAQs
- If I don't have a vacuum, how do I remove the liquid from my plate?
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If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.
- Should I perform the assay with the filter plates or with V-bottom plates?
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Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.
- After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?
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The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.
- What is the shelf life of LEGENDplex™ kits?
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LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.
- Is special software required for data analysis?
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Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.
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