The Evolution and Future of Single-Cell Proteogenomics

Providing higher multiplex phenotypic characterization over flow cytometry in addition to single-cell RNA sequencing (scRNA-seq), cellular indexing of transcriptomes and epitopes by sequencing (CITEseq) has huge potential to take single-cell biology to unprecedented new levels. An extremely powerful technique, thanks to its capacity to simultaneously combine protein and transcriptomic analysis in thousands of cells in parallel, CITE-seq affords enhanced understanding of a diversity of cellular processes. This is achieved using antibody-oligonucleotide conjugates to couple specific detection of protein markers with unbiased transcriptome profiling, opening up the possibility to identify novel cell types and shed new light on previously unseen events.

 

In this book you will learn about:

 

Simultaneous Proteomics and Transcriptomics

  • TotalSeq™ reagents enhance single-cell analysis.

Multiparameter Immunophenotyping by Single-Cell Sequencing

  • Using standardized oligonucleotide barcode antibody conjugates

Sample Multiplexing and Workflow Optimization in Single-Cell Genomics

  • The transforming power of TotalSeq™ hashtags

Utilities and Applications of TotalSeq™ tools

  • The voice of the scientific community

The Future of Precision Oncology

  • Proteogenomic advances are enabling an in-depth look inside tumor cells

 

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