Recombinant Human IL-31 (ELISA Std.)

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Regulatory Status
RUO
Other Names
Interleukin-31,
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Human_IL-31_RECOM_ELISA_032717
  • Human_IL-31_RECOM_ELISA_032717
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561901 4 pack $80.00
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Description

IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune responses. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.

Product Details
Technical data sheet

Product Details

Source
E. coli derived Ser24-Thr164.
Molecular Mass
The recombinant protein has a predicted molecular mass of approximately 15.8 kDa.
Purity
>95%, as determined by Coomassie stained SDS-PAGE
Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (please contact technical support for mass/vial, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt. Prior to use, reconstitute the lyophilized powder with 0.2 ml of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Use a manual defrost freezer and avoid repeated freeze thaw cycles.
Application

ELISA - Quality tested

Recommended Usage

Each lot of this protein is quality control tested by ELISA. For use as an ELISA standard, a standard curve comprised of doubling dilutions from 2000 pg/mL to 31.3 pg/mL is suggested. It is recommended that the reagent be titrated for optimal performance.

Application Notes

This IL-31 protein is useful as a standard for a human IL-31 sandwich ELISA, using unlabeled Poly5300 antibody as capture and biotinylated Poly5300 antibody  as detection.

Antigen Details

Structure
161 aa with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution

Secreted by activated CD4<sup+</sup> T cells.

Function
IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand/Receptor
Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Biology Area
Immunology, Innate Immunity, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References

1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.

Gene ID
386653 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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