Purified anti-Cytokeratin 18 Antibody (Previously Covance catalog# SIG-3466)

Pricing & Availability
Clone
5E8/CK-18 (See other available formats)
Regulatory Status
RUO
Other Names
CYK18, K18, CK-18, Keratin, type I cytoskeletal 18, CK-18, keratin-18, cytokeratin-18, cell proliferation-inducing protein 46
Previously
Covance Catalog# SIG-3466
Isotype
Mouse IgG2b
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Product Citations
publications
5E8_Purified_Cytokeratin-18_Antibody_IHC_030215
Immunostaining with Cytokeratin 18 showing cytoplasmic staining of the surface and crypt epithelium in normal human colon tissue.
  • 5E8_Purified_Cytokeratin-18_Antibody_IHC_030215
    Immunostaining with Cytokeratin 18 showing cytoplasmic staining of the surface and crypt epithelium in normal human colon tissue.
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914301 100 µL $374.00
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Description

Cytokeratin 18 is a member of the intermediate filament gene family. Cytokeratin 18 along with Cytokeratin 8 are perhaps the most common members of the intermediate filament family and are expressed in single layer epithelial tissues.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Purified Antibody (in 0.01M PBS + 0.1% NaN3 + 1% BSA).
Storage & Handling
When stored at ≤20°C, product is stable until the date shown on the label. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. If thawed and stored at 2-8°C, product is stable for 18 months from the date of thaw or until the expiry date on the label, whichever comes first.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: ≥1:250 for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: 1 mM EDTA in 20 mM Tris buffer pH 9.4
Incubation: 60 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry.

Positive Control Tissue: Normal human colon

Product Citations
RRID
AB_2565154 (BioLegend Cat. No. 914301)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3875 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Cytokeratin 18
Specificity Alt (DOES NOT SHOW ON TDS):
Cytokeratin 18
UniProt
View information about Cytokeratin 18 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/05/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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