Neurofilaments (NFs) belong to the intermediate filament family of proteins, and are primarily composed of three subunits; neurofilament light (NF-L), medium (NF-M), and heavy (NF-H). NFs are essential for providing structural support and maintenance of axon caliber. Filament assembly, function, and molecular interactions of neurofilaments can be regulated by phosphorylation. In Alzheimer’s disease (AD), aberrant phosphorylation of NF-M and NF-H leads to their accumulation in neuronal cell bodies, and disruption of NF transport to the axons. This has been attributed to decreased phosphatase and elevated NF kinase activity in the diseased brains. NFs can also be released from damaged or diseased neurons into blood or CSF. Therefore, elevated levels of NFs in the serum or CSF can serve as a biomarker for neuronal injury or degeneration.

 

BioLegend’s Neurofilament L/M/H Antibody Sampler Kit offers a great selection of highly validated antibodies for detection of neurofilament subunits. These antibodies are offered in Alexa Fluor® conjugated formats to allow multiplex immunofluorescence (IF) staining in tissue or cell culture. The kit components allow visualization of neuronal axons, dendrites, and cell bodies depending on the clone utilized.

 

Neurofilament Antibody Sampler Kit Contents:

Specificity

Clone

Format

Reactivity

Application

Neurofilament L (NF-L)

NFL3

Alexa Fluor® 647

Hu, Ms, Rat

IHC-P

Neurofilament H (NF-H), Nonphosphorylated

SMI 32

Alexa Fluor® 488

Hu, Ms, Rat

IHC-P

Neurofilament H (NF-H), Phosphorylated

SMI 31

Alexa Fluor® 594

Hu, Ms, Rat

IHC-P

Neurofilament H & M (NF-H/NF-M),

Hypophosphorylated

SMI 35

Alexa Fluor® 594

Hu, Ms, Rat

ICC

Neurofilament H & M (NF-H/NF-M),

Phosphorylated

SMI 310

Alexa Fluor® 488

Hu, Ms, Rat

IHC-P

Clone SMI 31 detects a phosphorylated epitope in extensively phosphorylated NF-H and primarily reacts with axons.

 

 

 

 

 

Clone SMI 32 detects a non-phosphorylated epitope in NF-H and visualizes neuronal cell bodies, axons, and dendrites.

 

 

 

 

 

Clone SMI 35 detects phosphorylated forms of NF-M and NF-H, and may be used to detect early neuronal cell pathology and intraneuronal neurofibrillary tangles in Alzheimer’s disease.

 

 

 

 

Clone SMI 310, similar to clone SMI 31, recognizes phosphorylated NF-H, and demonstrates strong reaction with extraneuronal (ghost) neurofibrillary tangles in AD. Clones SMI 35 and SMI 310 generally react with axons.

 

 

 

 

Clone NFL3 detects neurofilament light (NF-L) subunits, which can be a biomarker for axonal degeneration. Blood and cerebrospinal fluid levels of NF-L can be elevated during neurodegenerative diseases like Alzheimer’s.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account