Neurofilaments (NFs) belong to the intermediate filament family of proteins, and are primarily composed of three subunits; neurofilament light (NF-L), medium (NF-M), and heavy (NF-H). NFs are essential for providing structural support and maintenance of axon caliber. Filament assembly, function, and molecular interactions of neurofilaments can be regulated by phosphorylation. In Alzheimer’s disease (AD), aberrant phosphorylation of NF-M and NF-H leads to their accumulation in neuronal cell bodies, and disruption of NF transport to the axons. This has been attributed to decreased phosphatase and elevated NF kinase activity in the diseased brains. NFs can also be released from damaged or diseased neurons into blood or CSF. Therefore, elevated levels of NFs in the serum or CSF can serve as a biomarker for neuronal injury or degeneration.
BioLegend’s Neurofilament L/M/H Antibody Sampler Kit offers a great selection of highly validated antibodies for detection of neurofilament subunits. These antibodies are offered in Alexa Fluor® conjugated formats to allow multiplex immunofluorescence (IF) staining in tissue or cell culture. The kit components allow visualization of neuronal axons, dendrites, and cell bodies depending on the clone utilized.