Purified anti-TNF-α Antibody (Previously Covance catalog# MMS-146P)

Pricing & Availability
Clone
TNF 104C (See other available formats)
Other Names
Tumor necrosis factor, TNF-a, cachectin, APC1 protein, TNF, monocyte-derived, TNF, macrophage-derived, TNF superfamily, member 2, tumor necrosis factor alpha, tumor necrosis factor ligand superfamily member 2
Previously
Covance Catalog# MMS-146P
Isotype
Mouse IgG2a
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Product Citations
publications
TNF-104C_Purified_TNF-a_Antibody_IHC_021315
Western blot using TNF-alpha antibody (901201) showing Human specificity by detecting only the Human TNF-alpha peptide and not the Murine TNF-alpha.
  • TNF-104C_Purified_TNF-a_Antibody_IHC_021315
    Western blot using TNF-alpha antibody (901201) showing Human specificity by detecting only the Human TNF-alpha peptide and not the Murine TNF-alpha.
Cat # Size Price Quantity Avail. Save
901201 250 µl $300
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised using recombinant soluble human TNF-α. It recognizes both the native and denatured human TNF-α.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, FA, ELISA, FC, Neut

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
Western blot: 1:100
Functional Blocking: 1:100
Flow Cytometry: 1:100
ELISA: 1:100
 

Application Notes

This antibody is effective in functional blocking, ELISA and flow cytometry.
 

RRID
AB_2565002 (BioLegend Cat. No. 901201)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Signal Transduction
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/19/2016

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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