- Clone
- L42/17 (See other available formats)
- Other Names
- SynDIG1, Tmem90b, Synapse differentiation-inducing gene protein 1, Dispanin subfamily C member 2, Transmembrane protein 90B, DSPC2, C20orf39
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Western blot of anti-SynDIG1 antibody (clone L42/17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human frontal cortex lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of L42/17 or mouse IgG2a, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 851801 | 25 µg | $75 | ||||
| 851802 | 100 µg | $140 | ||||
SynDIG1 is a type II transmembrane protein, which has been reported to regulate synaptic AMPA receptor content in rat hippocampal neurons in an activity-dependent manner. It has been found to be co-localized with AMPA receptors at both post-synaptic terminals and extra-synaptic regions.
Product DetailsProduct Details
- Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- GST Fusion protein corresponding to the cytoplasmic N-terminus (a.a. 1-183) of mouse SynDIG1.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Reported in the literature - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is more reactive with mouse and rat lysates than human.
Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC)
-
Application References
(PubMed link indicates BioLegend citation) -
- Kalashnikova E, et al. 2010. Neuron. 65(1):80-93.
- RRID
-
AB_2728581 (BioLegend Cat. No. 851801)
AB_2728582 (BioLegend Cat. No. 851802)
Antigen Details
- Structure
- SynDIG1 (Tmem90b) is a 258 amino acid protein with an apparent molecular mass of ~ 29 kD.
- Distribution
-
Tissue distribution: Mainly in the Central Nervous System. Also expressed in muscle and reproductive organs.
Cellular distribution: Plasma Membrane and endosome. - Function
- SynDIG1 regulates synaptic AMPA receptor content at nascent synapses.
- Interaction
- SynDIG1 forms homodimers in vivo. It also interacts with GRIA1 and GRIA2.
- Biology Area
- Cell Biology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, Postsynaptic proteins
- Antigen References
-
- Sumioka A. 2013. J. Biochem. 153(4):331.
- Diaz E. 2012. J. Physiol. 590(1):33.
- Gene ID
- 79953 View all products for this Gene ID
- UniProt
- View information about SynDIG1 on UniProt.org
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