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Purified anti-SynDIG1 Antibody

Pricing & Availability
Clone
L42/17 (See other available formats)
Regulatory Status
RUO
Other Names
SynDIG1, Tmem90b, Synapse differentiation-inducing gene protein 1, Dispanin subfamily C member 2, Transmembrane protein 90B, DSPC2, C20orf39
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L42slash17_PURE_SynDIG1_Antibody_032718
Western blot of anti-SynDIG1 antibody (clone L42/17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human frontal cortex lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of L42/17 or mouse IgG2a, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • L42slash17_PURE_SynDIG1_Antibody_032718
    Western blot of anti-SynDIG1 antibody (clone L42/17). Lane 1: Molecular weight marker; Lane 2: 20 µg of human frontal cortex lysate; Lane 3: 20µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of L42/17 or mouse IgG2a, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
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851801 25 µg $89
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851802 100 µg $165
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Description

SynDIG1 is a type II transmembrane protein, which has been reported to regulate synaptic AMPA receptor content in rat hippocampal neurons in an activity-dependent manner. It has been found to be co-localized with AMPA receptors at both post-synaptic terminals and extra-synaptic regions.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST Fusion protein corresponding to the cytoplasmic N-terminus (a.a. 1-183) of mouse SynDIG1.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is more reactive with mouse and rat lysates than human.

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC)

Application References

(PubMed link indicates BioLegend citation)
  1. Kalashnikova E, et al. 2010. Neuron. 65(1):80-93.
RRID
AB_2728581 (BioLegend Cat. No. 851801)
AB_2728582 (BioLegend Cat. No. 851802)

Antigen Details

Structure
SynDIG1 (Tmem90b) is a 258 amino acid protein with an apparent molecular mass of ~ 29 kD.
Distribution

Tissue distribution: Mainly in the Central Nervous System. Also expressed in muscle and reproductive organs.
Cellular distribution: Plasma Membrane and endosome.

Function
SynDIG1 regulates synaptic AMPA receptor content at nascent synapses.
Interaction
SynDIG1 forms homodimers in vivo. It also interacts with GRIA1 and GRIA2.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, Postsynaptic proteins
Antigen References
  1. Sumioka A. 2013. J. Biochem. 153(4):331.
  2. Diaz E. 2012. J. Physiol. 590(1):33.
Gene ID
79953 View all products for this Gene ID
UniProt
View information about SynDIG1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 03/28/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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  • Purified anti-SynDIG1
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