Purified anti-SynCAM4 Antibody (Previously Covance catalog# MMS-5257)

Pricing & Availability
Clone
N244/5 (See other available formats)
Other Names
TSLL2, IGSF4C, Necl-4, Cell adhesion molecule 4, NECL-4, SynCAM 4, TSLC1-like 2, TSLC1-like protein 2, nectin-like protein 4, membrane glycoprotein Tsll2, immunoglobulin superfamily member 4C, immunoglobulin superfamily, member 4C
Previously
Covance Catalog# MMS-5257
Isotype
Mouse IgG1
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Product Citations
publications
N244-5_PURE_SynCam4_Antibody_1_WB_012618
Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 0.1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N244-5_PURE_SynCam4_Antibody_1_WB_012618
    Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20ug of rat brain membrane lysate. The blot was incubated with 0.1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • N244-5_PURE_SynCam4_Antibody_2_IHC_012618
    IHC staining of anti-SynCAM4 antibody (clone N244/5) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N244-5_PURE_SynCam4_Antibody_3_IHC_012618
    IHC staining of anti-SynCAM4 antibody (clone N244/5) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N244-5_PURE_SynCam4_Antibody_4_IHC_012618
    IHC staining of anti-SynCAM4 antibody (clone N244/5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
833302 25 µl $70.00
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833301 100 µl $140.00
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Description

SynCAM molecules mediate homotypic adhesion in the neural synapse.  These proteins are also known as Necl1-4 or cell adhesion molecule 1-4.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-321 (signal peptide and extracellular N-terminus) of mouse SynCAM4.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg per ml. Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

RRID
AB_2749915 (BioLegend Cat. No. 833302)
AB_2564965 (BioLegend Cat. No. 833301)

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Go To Top Version: 2    Revision Date: 06/23/2016

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